Rat anti-Mouse VEGF-B Monoclonal Antibody | anti-VEGF-B antibody

Rat Anti-Mouse VEGF-B

Cat. Num. AAA690939

• Gene Names: VEGFB; VRF; VEGFL
• Reactivity: Mouse
• Purity: Protein G affinity chromatography
• Synonyms: VEGF-B; Monoclonal Antibody; Rat Anti-Mouse VEGF-B; Anti-mouse VEGF-B (#6F72); anti-VEGF-B antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Specificity: This antibody recognizes mouse VEGF-B 186 but not 167. Cross reactivity to other species have not been tested!
• Purity/Purification: Protein G affinity chromatography
• Form/Format: The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at -7
• Sequence Length: 188

• Application Notes: 1. Western blot: Yes (1:400-1000) 2. Immunohistochemistry (Paraffin): Yes (1:50-100)
• Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with rat VEGF-B186 recombinant protein). The IgG2 fraction of culture supernatant was purified by Protein G affinity chromatography.
• Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.
• Preparation and Storage: Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Testing Data

Related Product Information for anti-VEGF-B antibody

Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity.

NCBI and Uniprot Product Information

• NCBI GI #: 344179103
• NCBI GeneID: 7423
• NCBI Accession #: NP_001230662.1
• NCBI GenBank Nucleotide #: NM_001243733.1
• UniProt Accession #: P49765; Q16528
• Molecular Weight: 21,602 Da
• NCBI Official Full Name: vascular endothelial growth factor B isoform VEGFB-167
• NCBI Official Synonym Full Names: vascular endothelial growth factor B
• NCBI Official Symbol: VEGFB
• NCBI Official Synonym Symbols: VRF; VEGFL
• NCBI Protein Information: vascular endothelial growth factor B; VEGF-related factor
• UniProt Protein Name: Vascular endothelial growth factor B
• UniProt Gene Name: VEGFB
• UniProt Synonym Gene Names: VRF; VEGF-B; VRF
• UniProt Entry Name: VEGFB_HUMAN

NCBI Description

This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011]

Uniprot Description

Function: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

Subunit structure: Homodimer; disulfide-linked. Can also form heterodimer with VEGF. Ref.5

Subcellular location: Secreted. Note: Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Tissue specificity: Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Post-translational modification: VEGF-B186 is O-glycosylated

By similarity.

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.