Typical Testing Data/Standard Curve (for reference only)
Rat Aryl hydrocarbon receptor ELISA Kit | Ahr elisa kit
Rat Aryl hydrocarbon receptor ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
binds 3-methylcholanthrene and other aromatic hydrocarbons; may play a role in bone formation [RGD, Feb 2006]
Uniprot Description
AHR: a nuclear receptor for aryl hydrocarbons involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals. AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes . AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke. Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits. Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1b). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes. AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism. Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer.
Protein type: Transcription factor; Nuclear receptor; DNA-binding
Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus
Molecular Function: aryl hydrocarbon receptor activity; DNA binding; Hsp90 protein binding; ligand-dependent nuclear receptor activity; protein binding; protein dimerization activity; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: apoptosis; B cell homeostasis; blood circulation; blood vessel development; blood vessel morphogenesis; blood vessel remodeling; camera-type eye development; cell morphogenesis; circadian regulation of gene expression; embryonic hemopoiesis; gland development; immune system process; intracellular receptor-mediated signaling pathway; liver development; lymphocyte homeostasis; negative regulation of systemic arterial blood pressure; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of vasoconstriction; ovarian follicle development; patterning of blood vessels; positive regulation of cell size; positive regulation of growth rate; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transcriptional preinitiation complex assembly; post-embryonic hemopoiesis; prostate gland development; regulation of B cell proliferation; regulation of blood vessel size; regulation of gene expression; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; reproductive structure development; response to estradiol stimulus; response to organic cyclic substance; response to toxin; response to xenobiotic stimulus; smooth muscle development; spleen development; T cell homeostasis; transcription from RNA polymerase II promoter
Research Articles on Ahr
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Product Notes
The Rat Ahr ahr (Catalog #AAA2882898) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2882898 ELISA Kit recognizes Rat Ahr. It is sometimes possible for the material contained within the vial of "Aryl hydrocarbon receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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