Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) Standard Curve.)
Rat TRNA pseudouridine Synthase A, Mitochondrial (PUS1) ELISA Kit | PUS1 elisa kit
Rat TRNA pseudouridine Synthase A, Mitochondrial (PUS1) ELISA Kit
Reactivity
Rat
Synonyms
TRNA pseudouridine Synthase A; Mitochondrial (PUS1); Rat TRNA pseudouridine Synthase A; Mitochondrial (PUS1) ELISA Kit; MGC11268; pseudouridine synthase 1; tRNA uridine isomerase I; PUS1 elisa kit
Reactivity
Rat
Specificity
Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PUS1. No significant cross-reactivity or interference between Rat PUS1 and analogues was observed.
Sequence Length
423
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) Standard Curve.)
Related Product Information for PUS1 elisa kit
Description: This Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) ELISA Kit employs a two-site sandwich ELISA to quantitate PUS1 in samples. An antibody specific for PUS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PUS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PUS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PUS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PUS1 converts uridine into pseudouridine after the nucleotide has been incorporated into RNA. Pseudouridine may have a functional role in tRNAs and may assist in the peptidyl transfer reaction of rRNAs PUS1-1 encodes a protein with an N-terminal mitochondrial leader peptide. Western blot analysis detected full-length PUS1-1 at an apparent molecular mass of 47 kD, and the mature protein, lacking the mitochondrial localization signal, was about 37 kD. The PUS1-2 and PUS1-3 transcripts differ in the 5-prime untranslated region but encode the same protein that begins with an internal methionine (met29) relative to PUS1-1. Immunofluorescence localized PUS1-1 to mitochondria and PUS1-2 and PUS1-3 to nuclei.
Background: PUS1 converts uridine into pseudouridine after the nucleotide has been incorporated into RNA. Pseudouridine may have a functional role in tRNAs and may assist in the peptidyl transfer reaction of rRNAs PUS1-1 encodes a protein with an N-terminal mitochondrial leader peptide. Western blot analysis detected full-length PUS1-1 at an apparent molecular mass of 47 kD, and the mature protein, lacking the mitochondrial localization signal, was about 37 kD. The PUS1-2 and PUS1-3 transcripts differ in the 5-prime untranslated region but encode the same protein that begins with an internal methionine (met29) relative to PUS1-1. Immunofluorescence localized PUS1-1 to mitochondria and PUS1-2 and PUS1-3 to nuclei.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tRNA pseudouridine synthase A
NCBI Official Synonym Full Names
pseudouridine synthase 1
NCBI Official Symbol
Pus1
NCBI Protein Information
tRNA pseudouridine synthase A; tRNA pseudouridine synthase A, mitochondrial
UniProt Protein Name
tRNA pseudouridine synthase A, mitochondrial
Protein Family
UniProt Gene Name
Pus1
UniProt Entry Name
TRUA_RAT
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Product Notes
The Rat PUS1 pus1 (Catalog #AAA9712543) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712543 ELISA Kit recognizes Rat PUS1. It is sometimes possible for the material contained within the vial of "TRNA pseudouridine Synthase A, Mitochondrial (PUS1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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