Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Target of EGR1 protein 1 (TOE1) Standard Curve.)
Mouse Target Of EGR1 Protein 1 (TOE1) ELISA Kit | TOE1 elisa kit
Mouse Target Of EGR1 Protein 1 (TOE1) ELISA Kit
Gene Names
Toe1; AI413517; 4930584N22Rik; 4933424D16Rik
Reactivity
Mouse
Synonyms
Target Of EGR1 Protein 1 (TOE1); Mouse Target Of EGR1 Protein 1 (TOE1) ELISA Kit; TOE1; FLJ13949; TOE1 elisa kit
Reactivity
Mouse
Specificity
Mouse Target of EGR1 protein 1 (TOE1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TOE1. No significant cross-reactivity or interference between Mouse TOE1 and analogues was observed.
Sequence Length
511
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Target of EGR1 protein 1 (TOE1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Target of EGR1 protein 1 (TOE1) Standard Curve.)
Related Product Information for TOE1 elisa kit
Description: This Mouse Target of EGR1 protein 1 (TOE1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOE1 in samples. An antibody specific for TOE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Toll interacting protein, also known as TOLLIP, is an inhibitory adaptor protein.It is an inhibitory adaptor protein within Toll-like receptors (TLR). The TLR pathway is a part of the innate immune system that recognizes structurally conserved molecular patterns of microbial pathogens, leading to an inflammatory immune response. Compared with the full-length isoform, 2 of the human proteins lack the N-terminal TBD, and 1 lacks the C2 domain. A mouse-specific isoform lacks the C-terminal CUE domain. EST database analysis suggested that full-length human TOLLIP is ubiquitously expressed. Expression of the other human variants appeared to be more restricted, with evidence for enrichment in brain.
Background: Toll interacting protein, also known as TOLLIP, is an inhibitory adaptor protein.It is an inhibitory adaptor protein within Toll-like receptors (TLR). The TLR pathway is a part of the innate immune system that recognizes structurally conserved molecular patterns of microbial pathogens, leading to an inflammatory immune response. Compared with the full-length isoform, 2 of the human proteins lack the N-terminal TBD, and 1 lacks the C2 domain. A mouse-specific isoform lacks the C-terminal CUE domain. EST database analysis suggested that full-length human TOLLIP is ubiquitously expressed. Expression of the other human variants appeared to be more restricted, with evidence for enrichment in brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
target of EGR1 protein 1
NCBI Official Synonym Full Names
target of EGR1, member 1 (nuclear)
NCBI Official Symbol
Toe1
NCBI Official Synonym Symbols
AI413517; 4930584N22Rik; 4933424D16Rik
NCBI Protein Information
target of EGR1 protein 1
UniProt Protein Name
Target of EGR1 protein 1
Protein Family
UniProt Gene Name
Toe1
UniProt Entry Name
TOE1_MOUSE
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Product Notes
The Mouse TOE1 toe1 (Catalog #AAA9716692) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716692 ELISA Kit recognizes Mouse TOE1. It is sometimes possible for the material contained within the vial of "Target Of EGR1 Protein 1 (TOE1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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