Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF9/4-1BB PicoKine ELISA Kit standard curve )
Mouse TNFRSF9/4-1BB ELISA Kit | TNFRSF9 elisa kit
Mouse TNFRSF9/4-1BB PicoKine ELISA Kit
Gene Names
Tnfrsf9; ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
Reactivity
Mouse
Synonyms
TNFRSF9/4-1BB; Mouse TNFRSF9/4-1BB PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily; member 9; 4-1BB; 4-1BB ligand receptor; CD137; CD137 antigen; CDw137; Homolog of mouse 4 1BB; ILA; Induced by lymphocyte activation (ILA); Interleukin activated receptor homolog of mouse Ly63; Receptor protein 4 1BB; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; TNR9_HUMAN; TNFRSF9 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse TNFRSF9
Sequence Length
256
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF9/4-1BB PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF9/4-1BB PicoKine ELISA Kit standard curve )
Related Product Information for TNFRSF9 elisa kit
Principle of the assay: mouse TNFRSF9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF9 has been precoated onto 96-well plates. Standards(NSO, V24-L187) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF9 amount of sample captured in plate.
Background: CD137 is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), 4-1BB and induced by lymphocyte activation (ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.
Background: CD137 is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), 4-1BB and induced by lymphocyte activation (ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
27,598 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 9
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 9
NCBI Official Symbol
Tnfrsf9
NCBI Official Synonym Symbols
ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
NCBI Protein Information
tumor necrosis factor receptor superfamily member 9; CD137 antigen; T-cell antigen 4-1BB; 4-1BB ligand receptor; secreted CD137 antigen
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 9
Protein Family
UniProt Gene Name
Tnfrsf9
UniProt Synonym Gene Names
Cd137; Ila; Ly63
UniProt Entry Name
TNR9_MOUSE
Uniprot Description
TNFRSF9: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.
Protein type: Membrane protein, integral; Receptor, misc.
Cellular Component: extracellular space; membrane; integral to membrane; external side of plasma membrane
Molecular Function: cytokine binding; receptor activity
Biological Process: regulation of cell proliferation
Research Articles on TNFRSF9
Similar Products
Product Notes
The Mouse TNFRSF9 tnfrsf9 (Catalog #AAA177234) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177234 ELISA Kit recognizes Mouse TNFRSF9. It is sometimes possible for the material contained within the vial of "TNFRSF9/4-1BB, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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