Hamster CD137 Monoclonal Antibody | anti-CD137 antibody

HAMSTER ANTI MOUSE CD137:Low Endotoxin

Cat. Num. AAA219724

• Gene Names: Tnfrsf9; ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
• Applications: Flow Cytometry, Functional Assay, Functional Assay
• Synonyms: CD137; Monoclonal Antibody; HAMSTER ANTI MOUSE CD137:Low Endotoxin; anti-CD137 antibody

• Host: Hamster
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 17B5
• Form/Format: Low Endotoxin; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 256

• Applicable Applications for anti-CD137 antibody: Flow cytometry (FC/FACS), Functional Assays (FN)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(ConA activated mouse spleen cells stained with hamster anti mouse CD137:FITC (MBS219735))

Testing Data

(ConA activated mouse spleen cells stained with hamster anti mouse CD137:Alexa Fluor ?488 (MBS219625A488))

Testing Data

(ConA activated mouse spleen cells stained with hamster anti mouse CD137:Alexa Fluor ?647 (MBS219625A647))

Related Product Information for anti-CD137 antibody

Hamster anti Mouse CD137 antibody, clone 17B5 recognizes mouse CD137, also known as 4-1BB ligand receptor, a member of the tumour necrosis factor superfamily. CD137 is a potent costimulatory receptor for the growth and functional maturation of activated T-cells in the presence of T-cell receptor signal. It is found on activated T-cells, NK cells, monocytes and dendritic cells. Signalling through CD137 triggers T-cell expansion, cytokine production and prevention of activation-induced death of effector T-cells. CD137-mediated activation is distinct from and independent of CD28. Antibody cross-linking of CD137 on NK cells stimulates the secretion of IFN-gamma, cellular proliferation, CD25 expression and responsiveness to IL-2 and is associated with tumour activity, but not cytotoxicity. Hamster anti Mouse CD137 antibody, clone 17B5 may be used to block CD137-mediated T-cell proliferation.

NCBI and Uniprot Product Information

• NCBI GI #: 117422439
• NCBI GeneID: 21942
• NCBI Accession #: NP_001070977.1
• NCBI GenBank Nucleotide #: NM_001077509.1
• UniProt Accession #: P20334
• Molecular Weight: 27,598 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 9 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 9
• NCBI Official Symbol: Tnfrsf9
• NCBI Official Synonym Symbols: ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor; CD137 antigen; T-cell antigen 4-1BB; secreted CD137 antigen
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 9
• UniProt Gene Name: Tnfrsf9
• UniProt Synonym Gene Names: Cd137; Ila; Ly63
• UniProt Entry Name: TNR9_MOUSE

Uniprot Description

TNFRSF9: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: extracellular space; membrane; integral to membrane; external side of plasma membrane

Molecular Function: cytokine binding; receptor activity

Biological Process: regulation of cell proliferation