Western Blot (WB)
(Figure 1. Western blot analysis of Tnfrsf18 using anti-Tnfrsf18 antibody.)
Rabbit anti-Mouse, Rat GITR/Tnfrsf18 Polyclonal Antibody | anti-Tnfrsf18 antibody
Anti-GITR/Tnfrsf18 Antibody
Gene Names
Tnfrsf18; AITR; Gitr
Reactivity
Mouse, Rat
Applications
Western Blot, ELISA
Purity
Immunogen affinity purified.
Synonyms
GITR/Tnfrsf18; Polyclonal Antibody; Anti-GITR/Tnfrsf18 Antibody; Tumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; CD357; Tnfrsf18; Gitr; anti-Tnfrsf18 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
228
Applicable Applications for anti-Tnfrsf18 antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml (Mouse, Rat)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived mouse GITR/Tnfrsf18 recombinant protein (Position: N41-T155).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of Tnfrsf18 using anti-Tnfrsf18 antibody.)
Western Blot (WB)
(Figure 1. Western blot analysis of Tnfrsf18 using anti-Tnfrsf18 antibody.)
Related Product Information for anti-Tnfrsf18 antibody
Description: Rabbit IgG polyclonal antibody for GITR/Tnfrsf18 detection. Tested with WB, Direct ELISA in Mouse; Rat.
Background: Tumor necrosis factor receptor superfamily member 18 (TNFRSF18) also known as activation-inducible TNFR family receptor (AITR) or glucocorticoid-induced TNFR-related protein (GITR) is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 4; 4 E2. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Background: Tumor necrosis factor receptor superfamily member 18 (TNFRSF18) also known as activation-inducible TNFR family receptor (AITR) or glucocorticoid-induced TNFR-related protein (GITR) is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 4; 4 E2. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
References
1. Grohmann, U., Volpi, C., Fallarino, F., Bozza, S., Bianchi, R., Vacca, C., Orabona, C., Belladonna, M. L., Ayroldi, E., Nocentini, G., Boon, L., Bistoni, F., Fioretti, M. C., Romani, L., Riccardi, C., Puccetti, P. Reverse signaling through GITR ligand enables dexamethasone to activate IDO in allergy. Nature Med. 13: 579-586, 2007.
2. Gurney, A. L., Marsters, S. A., Huang, A., Pitti, R. M., Mark, M., Baldwin, D. T., Gray, A. M., Dowd, P., Brush, J., Heldens, S., Schow, P., Goddard, A. D., Wood, W. I., Baker, K. P., Godowski, P. J., Ashkenazi, A. Identification of a new member of the tumor necrosis factor family and its receptor, a human ortholog of mouse GITR. Curr. Biol. 9: 215-218, 1999.
3. Ji, H., Liao, G., Faubion, W. A., Abadia-Molina, A. C., Cozzo, C., Laroux, F. S., Caton, A., Terhorst, C. Cutting edge: the natural ligand for glucocorticoid-induced TNF receptor-related protein abrogates regulatory T cell suppression. J. Immun. 172: 5823-5827, 2004.
2. Gurney, A. L., Marsters, S. A., Huang, A., Pitti, R. M., Mark, M., Baldwin, D. T., Gray, A. M., Dowd, P., Brush, J., Heldens, S., Schow, P., Goddard, A. D., Wood, W. I., Baker, K. P., Godowski, P. J., Ashkenazi, A. Identification of a new member of the tumor necrosis factor family and its receptor, a human ortholog of mouse GITR. Curr. Biol. 9: 215-218, 1999.
3. Ji, H., Liao, G., Faubion, W. A., Abadia-Molina, A. C., Cozzo, C., Laroux, F. S., Caton, A., Terhorst, C. Cutting edge: the natural ligand for glucocorticoid-induced TNF receptor-related protein abrogates regulatory T cell suppression. J. Immun. 172: 5823-5827, 2004.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 18
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 18
NCBI Official Symbol
Tnfrsf18
NCBI Official Synonym Symbols
AITR; Gitr
NCBI Protein Information
tumor necrosis factor receptor superfamily member 18
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 18
Protein Family
UniProt Gene Name
Tnfrsf18
UniProt Synonym Gene Names
Gitr
UniProt Entry Name
TNR18_MOUSE
Uniprot Description
TNFRSF18: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, cytokine; Apoptosis
Cellular Component: membrane; integral to plasma membrane; extracellular region; plasma membrane; integral to membrane; external side of plasma membrane
Molecular Function: protein binding
Biological Process: apoptosis
Research Articles on Tnfrsf18
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Product Notes
The Tnfrsf18 tnfrsf18 (Catalog #AAA1753067) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GITR/Tnfrsf18 Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GITR/Tnfrsf18 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Mouse, Rat) Direct ELISA: 0.1-0.5ug/ml (Mouse) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the Tnfrsf18 tnfrsf18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GITR/Tnfrsf18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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