TNFRSF1A active protein

TNFRSF1A Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122785

• Gene Names: Tnfrsf1a; FPF; p55; TNF-R; TNFAR; TNFRI; Tnfr1; p55-R; CD120a; TNF-R1; TNFR60; Tnfr-2; TNF-R-I; TNF-R55; TNFRp55; TNF-alphaR1; TNFalpha-R1
• Purity: >96% as determined by SDS-PAGE
• Synonyms: TNFRSF1A; TNFRSF1A Protein; Mouse; Recombinant (His Tag); Mouse TNFR1/CD120a/TNFRSF1A Protein (His Tag); CD120a Protein; FPF Protein; p55 Protein; p55-R Protein; TNF-alphaR1 Protein; TNF-R Protein; TNF-R-I Protein; TNF-R1 Protein; TNF-R55 Protein; TNFalpha-R1 Protein; TNFAR Protein; Tnfr-2 Protein; Tnfr1 Protein; TNFR60 Protein; TNFRI Protein; TNFRp55 Protein; tumor necrosis factor receptor superfamily member 1A; TNFRSF1A active protein

• Host: HEK293 Cells
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala212

• Species: Mouse
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized human TNFa at 10ug/mL (100uL/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.28ug/mL.; 3. Immobilized mouse TNFa at 10ug/mL (100uL/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.13ug/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 30
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse TNFRSF1A (NP_ 035739.2) extracellular domain (Met 1-Ala 212) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TNFRSF1A active protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1/TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-kappaB, mediate apoptosis, and regulate inflammation processes.

Product Categories/Family for TNFRSF1A active protein

Neuroinflammation; Death Receptor Signaling

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Cottin V, et al. (2002) Restricted localization of the TNF receptor CD120a to lipid rafts: a novel role for the death domain. The journal of immunology. 168: 4095-102.

NCBI and Uniprot Product Information

• NCBI GI #: 31560799
• NCBI GeneID: 21937
• NCBI Accession #: NP_035739.2
• NCBI GenBank Nucleotide #: NM_011609.4
• UniProt Accession #: P25118
• Molecular Weight: 50,130 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 1A
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 1a
• NCBI Official Symbol: Tnfrsf1a
• NCBI Official Synonym Symbols: FPF; p55; TNF-R; TNFAR; TNFRI; Tnfr1; p55-R; CD120a; TNF-R1; TNFR60; Tnfr-2; TNF-R-I; TNF-R55; TNFRp55; TNF-alphaR1; TNFalpha-R1
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1A; TNF receptor alpha chain; TNF-RI; TNF-alpha-R1; TNFR-I; p60; tumor necrosis factor receptor 1; tumor necrosis factor receptor type I
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1A
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Tnfrsf1a
• UniProt Synonym Gene Names: Tnfr-1; Tnfr1; TNF-R1; TNF-RI; TNFR-I
• UniProt Entry Name: TNR1A_MOUSE

Uniprot Description

TNF-R1: Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD. Various TRADD-interacting proteins such as TRAFS, RIPK1 and possibly FADD, are recruited to the complex by their association with TRADD. This complex activates at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4, BRE, FEM1B, GRB2, SQSTM1 and TRPC4AP. Interacts with HCV core protein. Interacts with human cytomegalovirus/HHV-5 protein UL138. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: Golgi apparatus; extracellular space; protein complex; cell surface; mitochondrion; integral to membrane; cytosol; lipid raft; Golgi membrane; membrane; axon; plasma membrane; synapse; intracellular; nucleus; receptor complex

Molecular Function: protein binding; tumor necrosis factor receptor activity; protease binding; protein complex binding; tumor necrosis factor binding

Biological Process: positive regulation of protein import into nucleus, translocation; protein heterooligomerization; apoptosis; cytokine and chemokine mediated signaling pathway; programmed cell death; defense response; signal transduction; positive regulation of tumor necrosis factor production; prostaglandin metabolic process; regulation of apoptosis; positive regulation of angiogenesis; positive regulation of tyrosine phosphorylation of Stat1 protein; cell surface receptor linked signal transduction; DNA damage response, signal transduction resulting in induction of apoptosis; tumor necrosis factor-mediated signaling pathway; negative regulation of inflammatory response; positive regulation of neuron apoptosis; defense response to bacterium; negative regulation of interleukin-6 production; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of apoptosis; positive regulation of inflammatory response