Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thioredoxin-related transmembrane protein 1 (TMX1) Standard Curve.)
Mouse Thioredoxin-Related Transmembrane Protein 1 (TMX1) ELISA Kit | TMX1 elisa kit
Mouse Thioredoxin-Related Transmembrane Protein 1 (TMX1) ELISA Kit
Gene Names
Tmx1; Txndc1; 2810425A04Rik
Reactivity
Mouse
Synonyms
Thioredoxin-Related Transmembrane Protein 1 (TMX1); Mouse Thioredoxin-Related Transmembrane Protein 1 (TMX1) ELISA Kit; TMX1; DKFZp564E1962; PDIA11; TMX; TXNDC; TXNDC1; protein disulfide isomerase family A; member 11; thioredoxin domain containing 1; thioredoxin domain-containing; TMX1 elisa kit
Reactivity
Mouse
Specificity
Mouse Thioredoxin-related transmembrane protein 1 (TMX1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMX1. No significant cross-reactivity or interference between Mouse TMX1 and analogues was observed.
Sequence Length
278
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thioredoxin-related transmembrane protein 1 (TMX1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thioredoxin-related transmembrane protein 1 (TMX1) Standard Curve.)
Related Product Information for TMX1 elisa kit
Description: This Mouse Thioredoxin-related transmembrane protein 1 (TMX1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMX1 in samples. An antibody specific for TMX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TXNDC1 is a thioredoxin (TXN)-related protein with disulfide reductase activity. The deduced 280-amino acid protein has a predicted molecular mass of 31.8 kD and contains an N-terminal signal sequence, a TRX-like domain with an active site sequence CPAC, and a transmembrane domain. Northern blot analysis detected a 2.5-kb transcript in all tissues examined, with highest expression in kidney, liver, placenta, and lung. Confocal microscopy of intact HEK293 cells expressing TXNDC1 and Western analysis of subcellular fractions localized TXNDC1 primarily in the microsomal fraction in an endoplasmic reticulum (ER)-like staining pattern characterized by a diffuse network-like labeling of the cytoplasm and nuclear rim.
Background: TXNDC1 is a thioredoxin (TXN)-related protein with disulfide reductase activity. The deduced 280-amino acid protein has a predicted molecular mass of 31.8 kD and contains an N-terminal signal sequence, a TRX-like domain with an active site sequence CPAC, and a transmembrane domain. Northern blot analysis detected a 2.5-kb transcript in all tissues examined, with highest expression in kidney, liver, placenta, and lung. Confocal microscopy of intact HEK293 cells expressing TXNDC1 and Western analysis of subcellular fractions localized TXNDC1 primarily in the microsomal fraction in an endoplasmic reticulum (ER)-like staining pattern characterized by a diffuse network-like labeling of the cytoplasm and nuclear rim.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thioredoxin-related transmembrane protein 1
NCBI Official Synonym Full Names
thioredoxin-related transmembrane protein 1
NCBI Official Symbol
Tmx1
NCBI Official Synonym Symbols
Txndc1; 2810425A04Rik
NCBI Protein Information
thioredoxin-related transmembrane protein 1
UniProt Protein Name
Thioredoxin-related transmembrane protein 1
Protein Family
UniProt Gene Name
Tmx1
UniProt Synonym Gene Names
Txndc; Txndc1
UniProt Entry Name
TMX1_MOUSE
Uniprot Description
TXNDC1: May participate in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyze dithiol-disulfide exchange reactions.
Protein type: Membrane protein, integral
Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; nucleolus; integral to membrane
Molecular Function: disulfide oxidoreductase activity; protein disulfide isomerase activity
Biological Process: protein folding; transport; cell redox homeostasis
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Product Notes
The Mouse TMX1 tmx1 (Catalog #AAA9716751) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716751 ELISA Kit recognizes Mouse TMX1. It is sometimes possible for the material contained within the vial of "Thioredoxin-Related Transmembrane Protein 1 (TMX1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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