Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thioredoxin-related transmembrane protein 4 (TMX4) Standard Curve.)
Human Thioredoxin-Related Transmembrane Protein 4 (TMX4) ELISA Kit | TMX4 elisa kit
Human Thioredoxin-Related Transmembrane Protein 4 (TMX4) ELISA Kit
Gene Names
TMX4; PDIA14; TXNDC13; DJ971N18.2
Reactivity
Human
Synonyms
Thioredoxin-Related Transmembrane Protein 4 (TMX4); Human Thioredoxin-Related Transmembrane Protein 4 (TMX4) ELISA Kit; TMX4; DJ971N18.2; KIAA1162; PDIA14; TXNDC13; protein disulfide isomerase family A; member 14; thioredoxin domain containing 13; TMX4 elisa kit
Reactivity
Human
Specificity
Human Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMX4. No significant cross-reactivity or interference between Human TMX4 and analogues was observed.
Sequence Length
349
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thioredoxin-related transmembrane protein 4 (TMX4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thioredoxin-related transmembrane protein 4 (TMX4) Standard Curve.)
Related Product Information for TMX4 elisa kit
Description: This Human Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit employs a two-site sandwich ELISA to quantitate TMX4 in samples. An antibody specific for TMX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMX4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TMX4, a type I transmembrane protein, was localized to the ER and possessed a Trx-like domain that faced the ER lumen. A maleimide alkylation assay showed that a catalytic CXXC motif in the TMX4 Trx-like domain underwent changes in its redox state depending on cellular redox conditions, and, in the normal state, most of the endogenous TMX4 existed in the oxidized form. The redox potential of this domain indicated that TMX4 could work as a reductase in the environment of the ER. TMX4 had no effect on the acceleration of ER-associated degradation. Because TMX4 interacted with calnexin and ERp57 by co-immunoprecipitation assay, the role of TMX4 may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER.
Background: TMX4, a type I transmembrane protein, was localized to the ER and possessed a Trx-like domain that faced the ER lumen. A maleimide alkylation assay showed that a catalytic CXXC motif in the TMX4 Trx-like domain underwent changes in its redox state depending on cellular redox conditions, and, in the normal state, most of the endogenous TMX4 existed in the oxidized form. The redox potential of this domain indicated that TMX4 could work as a reductase in the environment of the ER. TMX4 had no effect on the acceleration of ER-associated degradation. Because TMX4 interacted with calnexin and ERp57 by co-immunoprecipitation assay, the role of TMX4 may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thioredoxin-related transmembrane protein 4
NCBI Official Synonym Full Names
thioredoxin related transmembrane protein 4
NCBI Official Symbol
TMX4
NCBI Official Synonym Symbols
PDIA14; TXNDC13; DJ971N18.2
NCBI Protein Information
thioredoxin-related transmembrane protein 4
UniProt Protein Name
Thioredoxin-related transmembrane protein 4
Protein Family
UniProt Gene Name
TMX4
UniProt Synonym Gene Names
KIAA1162; TXNDC13; PSEC0095; UNQ475/PRO938
UniProt Entry Name
TMX4_HUMAN
NCBI Description
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, one transmembrane domain and C-terminal ASP/GLU-rich calcium binding domain. Unlike most members of this gene family, it lacks a C-terminal ER-retention sequence. The encoded protein has been shown to have reductase activity in vitro. [provided by RefSeq, Jan 2017]
Research Articles on TMX4
Similar Products
Product Notes
The Human TMX4 tmx4 (Catalog #AAA9713906) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713906 ELISA Kit recognizes Human TMX4. It is sometimes possible for the material contained within the vial of "Thioredoxin-Related Transmembrane Protein 4 (TMX4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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