Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thioredoxin (TRX) Standard Curve.)
Mouse Thioredoxin (TRX) ELISA Kit | TRX elisa kit
Mouse Thioredoxin (TRX) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thioredoxin (TRX) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thioredoxin (TRX) Standard Curve.)
Background: Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals.Thioredoxins are characterized at the level of their amino acid sequence by the presence of two vicinal cysteines in a CXXC motif. These two cysteines are the key to the ability of thioredoxin to reduce other proteins. Thioredoxin proteins also have a characteristic tertiary structure termed the thioredoxin fold.The thioredoxins are kept in the reduced state by the flavoenzyme thioredoxin reductase, in a NADPH-dependent reaction. Thioredoxins act as electron donors to peroxidases and ribonucleotide reductase.
NCBI and Uniprot Product Information
Uniprot Description
TXN: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA- binding activity in a redox-dependent manner. Up-regulated by ionizing radiation. Belongs to the thioredoxin family.
Protein type: Nuclear receptor co-regulator
Cellular Component: cell soma; mitochondrion; axon; cytoplasm; dendrite; extracellular region; nucleus; cytosol
Molecular Function: protein binding; enzyme binding; protein disulfide oxidoreductase activity; peptide disulfide oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor
Biological Process: sulfate assimilation; response to radiation; negative regulation of protein export from nucleus; transcription, DNA-dependent; protein folding; regulation of transcription, DNA-dependent; cell redox homeostasis; transport; regulation of protein import into nucleus, translocation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; glycerol ether metabolic process; positive regulation of DNA binding
Research Articles on TRX
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Product Notes
The Mouse TRX txn (Catalog #AAA9716655) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716655 ELISA Kit recognizes Mouse TRX. It is sometimes possible for the material contained within the vial of "Thioredoxin (TRX), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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