Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) Standard Curve.)
Mouse Thioredoxin-Dependent Peroxide Reductase, Mitochondrial (PRDX3) ELISA Kit | PRDX3 elisa kit
Mouse Thioredoxin-Dependent Peroxide Reductase, Mitochondrial (PRDX3) ELISA Kit
Gene Names
Prdx3; Aop1; Ef2l; Mer5; Prx3; SP22; TDXM; D0Tohi1; AW822249
Reactivity
Mouse
Synonyms
Thioredoxin-Dependent Peroxide Reductase; Mitochondrial (PRDX3); Mouse Thioredoxin-Dependent Peroxide Reductase; Mitochondrial (PRDX3) ELISA Kit; PRDX3; AOP-1; AOP1; MER5; MGC104387; MGC24293; PRO1748; SP-22; antioxidant protein 1; thioredoxin-dependent peroxide reductase; PRDX3 elisa kit
Reactivity
Mouse
Specificity
Mouse Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRDX3. No significant cross-reactivity or interference between Mouse PRDX3 and analogues was observed.
Sequence Length
257
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) Standard Curve.)
Related Product Information for PRDX3 elisa kit
Description: This Mouse Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDX3 in samples. An antibody specific for PRDX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRDX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PRDX3 encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene.
Background: PRDX3 encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Thioredoxin-dependent peroxide reductase, mitochondrial
NCBI Official Synonym Full Names
peroxiredoxin 3
NCBI Official Symbol
Prdx3
NCBI Official Synonym Symbols
Aop1; Ef2l; Mer5; Prx3; SP22; TDXM; D0Tohi1; AW822249
NCBI Protein Information
thioredoxin-dependent peroxide reductase, mitochondrial
UniProt Protein Name
Thioredoxin-dependent peroxide reductase, mitochondrial
Protein Family
UniProt Gene Name
Prdx3
UniProt Synonym Gene Names
Aop1; Mer5; AOP-1
UniProt Entry Name
PRDX3_MOUSE
Uniprot Description
PRDX3: Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Belongs to the AhpC/TSA family.
Protein type: EC 1.11.1.15; Oxidoreductase
Cellular Component: mitochondrion; early endosome; cytoplasm; IkappaB kinase complex; cytosol; myelin sheath
Molecular Function: antioxidant activity; protein C-terminus binding; identical protein binding; peroxidase activity; thioredoxin peroxidase activity; peroxiredoxin activity; caspase inhibitor activity; oxidoreductase activity; protein kinase binding; kinase binding
Biological Process: negative regulation of kinase activity; mitochondrion organization and biogenesis; response to lipopolysaccharide; negative regulation of caspase activity; activation of NF-kappaB transcription factor; peptidyl-cysteine oxidation; response to hydrogen peroxide; regulation of mitochondrial membrane potential; hydrogen peroxide catabolic process; positive regulation of cell proliferation; myeloid cell differentiation; response to oxidative stress; negative regulation of neuron apoptosis; negative regulation of apoptosis; maternal placenta development
Research Articles on PRDX3
Similar Products
Product Notes
The Mouse PRDX3 prdx3 (Catalog #AAA9717302) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717302 ELISA Kit recognizes Mouse PRDX3. It is sometimes possible for the material contained within the vial of "Thioredoxin-Dependent Peroxide Reductase, Mitochondrial (PRDX3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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