Chicken Peroxiredoxin 3 Polyclonal Antibody | anti-PRDX3 antibody

Peroxiredoxin 3 (Antioxidant Protein 1, PRDX3, Thioredoxin Dependent Peroxide Reductase)

Cat. Num. AAA614338

• Gene Names: PRDX3; AOP1; MER5; AOP-1; SP-22; PRO1748; MGC24293; MGC104387
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Peroxiredoxin 3; Polyclonal Antibody; Peroxiredoxin 3 (Antioxidant Protein 1; PRDX3; Thioredoxin Dependent Peroxide Reductase); Anti -Peroxiredoxin 3 (Antioxidant Protein 1; anti-PRDX3 antibody

• Host: Chicken
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes human Peroxiredoxin 3. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Glycerol free.

• Applicable Applications for anti-PRDX3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:2000
• Immunogen: Synthetic peptide corresponding to aa1-256
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot: E coli-derived fusion protein as test antigen. Affi-anti- SAR1 IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)

Related Product Information for anti-PRDX3 antibody

Peroxiredoxin 3 has antioxidant function and is localized in the mitochondrion. The gene encoding this protein shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions.

Product Categories/Family for anti-PRDX3 antibody

Antibodies; Abs to Enzymes; Peroxiredoxin

NCBI and Uniprot Product Information

• NCBI GI #: 114205468
• NCBI GeneID: 10935
• UniProt Accession #: P30048; P35690; Q0D2H1; Q13776; Q5T5V2; Q96HK4; B2R7Z0; D3DRC9; Q14579; Q53HC2; Q9UHU0
• Molecular Weight: 27,693 Da
• NCBI Official Full Name: Peroxiredoxin 3
• NCBI Official Synonym Full Names: peroxiredoxin 3
• NCBI Official Symbol: PRDX3
• NCBI Official Synonym Symbols: AOP1; MER5; AOP-1; SP-22; PRO1748; MGC24293; MGC104387
• NCBI Protein Information: thioredoxin-dependent peroxide reductase, mitochondrial; HBC189; prx-III; peroxiredoxin-3; peroxiredoxin III; OTTHUMP00000020590; protein MER5 homolog; antioxidant protein 1
• UniProt Protein Name: Thioredoxin-dependent peroxide reductase, mitochondrial
• Protein Family: Thioredoxin-dependent peroxide reductase
• UniProt Gene Name: PRDX3
• UniProt Synonym Gene Names: AOP1
• UniProt Entry Name: PRDX3_HUMAN

NCBI Description

This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

PRDX3: Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Belongs to the AhpC/TSA family.

Protein type: Oxidoreductase; EC 1.11.1.15

Chromosomal Location of Human Ortholog: 10q25-q26

Cellular Component: mitochondrion; mitochondrial matrix; early endosome; cytoplasm; IkappaB kinase complex; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; thioredoxin peroxidase activity; caspase inhibitor activity; alkyl hydroperoxide reductase activity; kinase binding; protein kinase binding

Biological Process: mitochondrion organization and biogenesis; negative regulation of kinase activity; response to lipopolysaccharide; negative regulation of caspase activity; activation of NF-kappaB transcription factor; response to reactive oxygen species; peptidyl-cysteine oxidation; regulation of mitochondrial membrane potential; response to hydrogen peroxide; hydrogen peroxide catabolic process; positive regulation of cell proliferation; myeloid cell differentiation; response to oxidative stress; negative regulation of apoptosis; maternal placenta development