Rabbit anti-Human Osteonectin Polyclonal Antibody | anti-ON antibody

Osteonectin, CT (SPARC, Secreted Protein Acidic And Rich In Cysteine, ON, Basement-membrane Protein 40, BM-40, ON) (AP)

Cat. Num. AAA6491211

• Gene Names: SPARC; ON; OI17; BM-40
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Osteonectin; Polyclonal Antibody; CT (SPARC; Secreted Protein Acidic And Rich In Cysteine; ON; Basement-membrane Protein 40; BM-40; ON) (AP); anti-ON antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SPARC.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ON antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human SPARC (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SPARC Antibody (C-term) in Y79 cell line lysates (35ug/lane). SPARC (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of SPARC (arrow) using rabbit polyclonal SPARC Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skin tissue reacted withMBS6013553, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of widr cells using O8063-05B(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ON antibody

Antibodies; Osteonectin

References

1. Said, N., Oncogene 28(39), 3487-3498 (2009). 2. Maloney, S.C., Anticancer Res. 29(8), 3059-3064 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4507171
• NCBI GeneID: 6678
• NCBI Accession #: NP_003109
• NCBI GenBank Nucleotide #: NM_003118
• UniProt Accession #: P09486
• Molecular Weight: 35kDa
• NCBI Official Full Name: SPARC isoform 1
• NCBI Official Synonym Full Names: secreted protein acidic and cysteine rich
• NCBI Official Symbol: SPARC
• NCBI Official Synonym Symbols: ON; OI17; BM-40
• NCBI Protein Information: SPARC
• UniProt Protein Name: SPARC
• UniProt Gene Name: SPARC
• UniProt Synonym Gene Names: ON; BM-40; ON
• UniProt Entry Name: SPRC_HUMAN

NCBI Description

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]

Uniprot Description

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: extracellular space; platelet alpha granule membrane; nuclear matrix; cell surface; mitochondrion; cytoplasm; plasma membrane; extracellular region; basement membrane

Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding

Biological Process: response to peptide hormone stimulus; receptor-mediated endocytosis; response to gravity; platelet activation; extracellular matrix organization and biogenesis; ossification; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; platelet degranulation; response to cadmium ion; response to ethanol; response to cytokine stimulus; response to lead ion; negative regulation of endothelial cell proliferation; regulation of cell morphogenesis; response to calcium ion; blood coagulation; inner ear development; lung development

Disease: Osteogenesis Imperfecta, Type Xvii