Western Blot (WB)
(Western Blot analysis of Rat brain lysates showing detection of Synaptotagmin 7 protein using Mouse Anti-Synaptotagmin 7 Monoclonal Antibody, Clone S275-14. Primary Antibody: Mouse Anti-Synaptotagmin 7 Monoclonal Antibody at 1:100, 1:250, 1:500, and 1:1000.)
Mouse anti-Rat, Mouse Synaptotagmin 7 Monoclonal Antibody | anti-Syt7 antibody
Synaptotagmin-7 Antibody
Gene Names
Syt7; AI851541; B230112P13Rik
Reactivity
Rat, Mouse
Applications
Immunocytochemistry, Western Blot, Immunohistochemistry
Synonyms
Synaptotagmin 7; Monoclonal Antibody; Synaptotagmin-7 Antibody; IPCA-7; PCANAP7; Prostate cancer-associated protein 7; SYT-7; SYT7; Synaptotagmin VII; SytVII; anti-Syt7 antibody
Host
Mouse
Reactivity
Rat, Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
S275-14
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
403
Applicable Applications for anti-Syt7 antibody
Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
1:1000 WB
Certificate of Analysis
1 ug/mL was sufficient for detection of Synaptotagmin-7 in 20ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western Blot analysis of Rat brain lysates showing detection of Synaptotagmin 7 protein using Mouse Anti-Synaptotagmin 7 Monoclonal Antibody, Clone S275-14. Primary Antibody: Mouse Anti-Synaptotagmin 7 Monoclonal Antibody at 1:100, 1:250, 1:500, and 1:1000.)
Western Blot (WB)
(Western Blot analysis of Rat brain lysates showing detection of Synaptotagmin 7 protein using Mouse Anti-Synaptotagmin 7 Monoclonal Antibody, Clone S275-14. Primary Antibody: Mouse Anti-Synaptotagmin 7 Monoclonal Antibody at 1:100, 1:250, 1:500, and 1:1000.)
Related Product Information for anti-Syt7 antibody
Background Info: Detects ~45kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at ~65kD.
Scientific Background: Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid
Scientific Background: Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid
Product Categories/Family for anti-Syt7 antibody
References
1. Schengrund C.L., et al. (2002) J Biol Chem. 277: 32815. 2. Reichardt L.F., et al. (1981) J Cell Biol. 91:257.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,472 Da
NCBI Official Full Name
synaptotagmin-7 alpha isoform
NCBI Official Synonym Full Names
synaptotagmin VII
NCBI Official Symbol
Syt7
NCBI Official Synonym Symbols
AI851541; B230112P13Rik
NCBI Protein Information
synaptotagmin-7; sytVII; synaptotagmin 7
UniProt Protein Name
Synaptotagmin-7
Protein Family
UniProt Gene Name
Syt7
UniProt Synonym Gene Names
SytVII
UniProt Entry Name
SYT7_MOUSE
Uniprot Description
SYT7: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Belongs to the synaptotagmin family.
Protein type: Membrane protein, integral
Cellular Component: membrane; lysosome; integral to membrane; plasma membrane; synapse; cytoplasmic vesicle; cell junction
Molecular Function: clathrin binding; syntaxin binding; calcium-dependent phospholipid binding; metal ion binding; calcium ion binding
Biological Process: vesicle fusion; synaptic vesicle exocytosis; exocytosis; plasma membrane repair; calcium ion-dependent exocytosis of neurotransmitter; regulation of calcium ion-dependent exocytosis
Research Articles on Syt7
Similar Products
Product Notes
The Syt7 syt7 (Catalog #AAA802654) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Synaptotagmin-7 Antibody reacts with Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Synaptotagmin 7 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC). 1:1000 WB. Researchers should empirically determine the suitability of the Syt7 syt7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Synaptotagmin 7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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