Mouse Superoxide Dismutase 2, Mitochondrial (SOD2) ELISA Kit | SOD2 elisa kit

Mouse Superoxide Dismutase 2, Mitochondrial (SOD2)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA2125233

• Gene Names: Sod2; MnSOD; Sod-2
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Superoxide Dismutase 2; Mitochondrial (SOD2); Mouse Superoxide Dismutase 2; Mitochondrial (SOD2)(Sandwich ELISA) ELISA Kit; ELISA Kit for Superoxide Dismutase 2; SOD2/Superoxide Dismutase 2; Mitochondrial; IPO-B; MNSOD; Mn-SOD; SOD2 elisa kit

• Reactivity: Mouse
• Specificity: Reactive with Mouse Superoxide Dismutase 2, Mitochondrial (SOD2); This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase 2, Mitochondrial (SOD2). No significant cross-reactivity or interference between Superoxide Dismutase 2, Mitochondrial (SOD2) and analogues was observed.

• Applicable Applications for SOD2 elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Sandwich ELISA
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: 1.34ng/mL
• Detection Method: Double-antibody Sandwich
• Assay Length: 3 hours
• Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase 2, Mitochondrial (SOD2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase 2, Mitochondrial (SOD2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100.; Intra-Assay: CV<10%.; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase, Mitochondrial 1A (CKMT1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Creatine Kinase, Mitochondrial 1A (CKMT1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Creatine Kinase, Mitochondrial 1A (CKMT1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Creatine Kinase, Mitochondrial 1A (CKMT1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C; 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 50ul Stop Solution. Read at 450nm immediately.
• Preparation and Storage: Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life); The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for SOD2 elisa kit

Cardiovascular biology

NCBI and Uniprot Product Information

• NCBI GI #: 31980762
• NCBI GeneID: 20656
• NCBI Accession #: NP_038699.2
• NCBI GenBank Nucleotide #: NM_013671.3
• UniProt Accession #: P09671; Q64670; Q8VEM5
• Molecular Weight: 24,603 Da
• NCBI Official Full Name: superoxide dismutase
• NCBI Official Synonym Full Names: superoxide dismutase 2, mitochondrial
• NCBI Official Symbol: Sod2
• NCBI Official Synonym Symbols: MnSOD; Sod-2
• NCBI Protein Information: superoxide dismutase [Mn], mitochondrial
• UniProt Protein Name: Superoxide dismutase [Mn], mitochondrial
• Protein Family: Superoxide dismutase
• UniProt Gene Name: Sod2
• UniProt Synonym Gene Names: Sod-2
• UniProt Entry Name: SODM_MOUSE

Uniprot Description

SOD2: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the iron/manganese superoxide dismutase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.15.1.1; Mitochondrial; Oxidoreductase

Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrion; myelin sheath

Molecular Function: DNA binding; enzyme binding; identical protein binding; manganese ion binding; oxidoreductase activity; oxygen binding; superoxide dismutase activity

Biological Process: acetylcholine vasodilation involved in regulation of systemic arterial blood pressure; age-dependent response to oxidative stress; age-dependent response to reactive oxygen species; apoptotic mitochondrial changes; detection of oxygen; DNA damage response, signal transduction resulting in induction of apoptosis; erythrophore differentiation; glutathione metabolic process; heart development; hemopoiesis; hydrogen peroxide biosynthetic process; hydrogen peroxide metabolic process; induction of apoptosis by oxidative stress; iron ion homeostasis; liver development; locomotory behavior; mitochondrion organization and biogenesis; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of fat cell differentiation; negative regulation of fibroblast proliferation; negative regulation of neuron apoptosis; neuron development; oxygen homeostasis; positive regulation of nitric oxide biosynthetic process; post-embryonic development; protein homooligomerization; protein homotetramerization; regulation of blood pressure; regulation of catalytic activity; regulation of mitochondrial membrane potential; regulation of transcription from RNA polymerase II promoter; release of cytochrome c from mitochondria; removal of superoxide radicals; response to activity; response to axon injury; response to drug; response to gamma radiation; response to hydrogen peroxide; response to hyperoxia; response to nutrient levels; response to oxidative stress; response to reactive oxygen species; response to superoxide; sensory perception of sound; superoxide metabolic process; superoxide release; vasodilation