Rabbit YTHDC2 Polyclonal Antibody | anti-YTHDC2 antibody

anti-YTHDC2 antibody

Cat. Num. AAA7611958

• Gene Names: YTHDC2; CAHL
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE. Immunogen affinity purified
• Synonyms: YTHDC2; Polyclonal Antibody; anti-YTHDC2 antibody; CAHL

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE. Immunogen affinity purified
• Form/Format: Liquid. PBS with 0.02% sodium azide and 50% glycerol pH7.3

• Applicable Applications for anti-YTHDC2 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: 3'-5' RNA helicase YTHDC2
• Preparation and Storage: Store at -20 degree C for 12 months. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent analysis of NIH/3T3 cells using MBS7611958(YTHDC2 antibody) at dilution of 1:50.)

Western Blot (WB)

(rat testis tissue were subjected to SDS PAGE followed by western blot with MBS7611958(YTHDC2 antibody) at dilution of 1:1000)

Related Product Information for anti-YTHDC2 antibody

This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line.

NCBI and Uniprot Product Information

• NCBI GI #: 269847874
• NCBI GeneID: 64848
• NCBI Accession #: NP_073739.3
• NCBI GenBank Nucleotide #: NM_022828.4
• UniProt Accession #: Q9H6S0; B2RP66
• Molecular Weight: 160,248 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase YTHDC2 isoform 1
• NCBI Official Synonym Full Names: YTH domain containing 2
• NCBI Official Symbol: YTHDC2
• NCBI Official Synonym Symbols: CAHL
• NCBI Protein Information: probable ATP-dependent RNA helicase YTHDC2
• UniProt Protein Name: Probable ATP-dependent RNA helicase YTHDC2
• Protein Family: YTH domain-containing protein
• UniProt Gene Name: YTHDC2

NCBI Description

This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line. [provided by RefSeq, Sep 2016]

Uniprot Description

YTHDC2: Belongs to the DEAD box helicase family. DEAH subfamily.

Protein type: EC 3.6.4.13; RNA-binding

Chromosomal Location of Human Ortholog: 5q22.2

Cellular Component: endoplasmic reticulum

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; N6-methyladenosine-containing RNA binding; protein binding; RNA binding; RNA polymerase binding; RNA-dependent ATPase activity

Biological Process: response to tumor necrosis factor; RNA processing