Superoxide Dismutase 2, Mitochondrial (SOD2) Recombinant Protein | SOD2 recombinant protein

Recombinant Superoxide Dismutase 2, Mitochondrial (SOD2)

Cat. Num. AAA2010261

• Gene Names: Sod2; MnSOD; Sod-2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Superoxide Dismutase 2; Mitochondrial (SOD2); Recombinant Superoxide Dismutase 2; SOD2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-KHSLPD LPYDYGALEP HINAQIMQLH HSKHHAAYVN NLNATEEKYH EALAKGDVTT QVALQPALKF NGGGHINHTI FWTNLSPKGG GEPKGELLEA IKRDFGSFEK FKEKLTAVSV GVQGSGWGWL GFNKEQGRLQ IAACSNQDPL QGTTGLIPLL GIDVWEHAYY LQYKNVRPDY LKAIWNVINW ENVTERYTAC KK
• Sequence Length: 222

• Applicable Applications for SOD2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Lys25~Lys222 (Accession # P09671) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31980762
• NCBI GeneID: 20656
• NCBI Accession #: NP_038699.2
• NCBI GenBank Nucleotide #: NM_013671.3
• UniProt Accession #: P09671; Q64670; Q8VEM5
• Molecular Weight: 23.5kDa
• NCBI Official Full Name: superoxide dismutase
• NCBI Official Synonym Full Names: superoxide dismutase 2, mitochondrial
• NCBI Official Symbol: Sod2
• NCBI Official Synonym Symbols: MnSOD; Sod-2
• NCBI Protein Information: superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; manganese SOD; manganese superoxide dismutase
• UniProt Protein Name: Superoxide dismutase [Mn], mitochondrial
• Protein Family: Superoxide dismutase
• UniProt Gene Name: Sod2
• UniProt Synonym Gene Names: Sod-2
• UniProt Entry Name: SODM_MOUSE

Uniprot Description

SOD2: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the iron/manganese superoxide dismutase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Oxidoreductase; EC 1.15.1.1

Cellular Component: mitochondrion; cytoplasm; mitochondrial inner membrane; intracellular; myelin sheath

Molecular Function: identical protein binding; protein binding; DNA binding; manganese ion binding; metal ion binding; superoxide dismutase activity; oxidoreductase activity; oxygen binding

Biological Process: oxygen homeostasis; positive regulation of nitric oxide biosynthetic process; removal of superoxide radicals; heart development; locomotory behavior; vasodilation; age-dependent response to oxidative stress; post-embryonic development; protein homotetramerization; negative regulation of cell proliferation; apoptotic mitochondrial changes; sensory perception of sound; glutathione metabolic process; acetylcholine vasodilation involved in regulation of systemic arterial blood pressure; regulation of mitochondrial membrane potential; regulation of catalytic activity; regulation of blood pressure; response to gamma radiation; hemopoiesis; negative regulation of neuron apoptosis; response to axon injury; protein homooligomerization; response to drug; response to nutrient levels; erythrophore differentiation; mitochondrion organization and biogenesis; release of cytochrome c from mitochondria; response to superoxide; superoxide metabolic process; negative regulation of fat cell differentiation; liver development; regulation of transcription from RNA polymerase II promoter; response to reactive oxygen species; iron ion homeostasis; response to hyperoxia; response to hydrogen peroxide; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of fibroblast proliferation; detection of oxygen; age-dependent response to reactive oxygen species; hydrogen peroxide metabolic process; neuron development; response to activity; response to oxidative stress; superoxide release; induction of apoptosis by oxidative stress; hydrogen peroxide biosynthetic process; negative regulation of apoptosis