Mouse Spondin-1 (SPON1) ELISA Kit | SPON1 elisa kit

Mouse Spondin-1 (SPON1) ELISA Kit

Cat. Num. AAA7230117

• Gene Names: SPON1; f-spondin; VSGP/F-spondin
• Reactivity: Mouse
• Synonyms: Spondin-1 (SPON1); Mouse Spondin-1 (SPON1) ELISA Kit; SPON1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of SPON-1. No significant cross-reactivity or interference between SPON-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SPON-1 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for SPON1 elisa kit

Intended Uses: This SPON-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SPON-1. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: SPON-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SPON-1 antibody and an SPON-1-HRP conjugate. The assay sample and buffer are incubated together with SPON-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPON-1 concentration since SPON-1 from samples and SPON-1-HRP conjugate compete for the anti-SPON-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SPON-1 from the sample, fewer sites are left to bind SPON-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPON-1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SPON1 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 110347423
• NCBI GeneID: 10418
• NCBI Accession #: NP_006099.2
• NCBI GenBank Nucleotide #: NM_006108.3
• Molecular Weight: 90,973 Da
• NCBI Official Full Name: spondin-1
• NCBI Official Synonym Full Names: spondin 1, extracellular matrix protein
• NCBI Official Symbol: SPON1
• NCBI Official Synonym Symbols: f-spondin; VSGP/F-spondin
• NCBI Protein Information: spondin-1; spondin 1, (f-spondin) extracellular matrix protein; vascular smooth muscle cell growth-promoting factor
• UniProt Protein Name: Spondin-1
• Protein Family: Spondin
• UniProt Gene Name: SPON1
• UniProt Synonym Gene Names: KIAA0762; VSGP
• UniProt Entry Name: SPON1_HUMAN

Uniprot Description

SPON1: Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; endoplasmic reticulum lumen

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; post-translational protein modification; cell adhesion