Human spondin 1, extracellular matrix protein ELISA Kit | SPON1 elisa kit

Human Spondin-1, SPON1 ELISA Kit

Cat. Num. AAA932153

• Gene Names: SPON1; f-spondin; VSGP/F-spondin
• Reactivity: Human
• Synonyms: spondin 1; extracellular matrix protein; Human Spondin-1; SPON1 ELISA Kit; Human Spondin-1 (SPON1) ELISA kit; KIAA0762; MGC10724; f-spondin; VSGP/F-spondin; (f-spondin) extracellular matrix protein; SPON1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human spondin-1. No significant cross-reactivity or interference between human spondin-1 and analogues was observed.
• Sequence Length: 807

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml -10 ng/ml
• Sensitivity: 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPON1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for spondin-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any spondin-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for spondin-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of spondin-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 110347423
• NCBI GeneID: 10418
• NCBI Accession #: NP_006099.2
• NCBI GenBank Nucleotide #: NM_006108.3
• UniProt Accession #: Q9HCB6; O94862; Q8NCD7; Q8WUR5; A8K6W5
• Molecular Weight: 90,973 Da
• NCBI Official Full Name: spondin-1
• NCBI Official Synonym Full Names: spondin 1, extracellular matrix protein
• NCBI Official Symbol: SPON1
• NCBI Official Synonym Symbols: f-spondin; VSGP/F-spondin
• NCBI Protein Information: spondin-1; spondin 1, (f-spondin) extracellular matrix protein; vascular smooth muscle cell growth-promoting factor
• UniProt Protein Name: Spondin-1
• Protein Family: Spondin
• UniProt Gene Name: SPON1
• UniProt Synonym Gene Names: KIAA0762; VSGP
• UniProt Entry Name: SPON1_HUMAN

Uniprot Description

SPON1: Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; endoplasmic reticulum lumen

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; post-translational protein modification; cell adhesion