Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Serine/threonine-protein kinase PLK1 (PLK1) Standard Curve.)
Mouse Serine/Threonine-Protein Kinase PLK1 (PLK1) ELISA Kit | PLK1 elisa kit
Mouse Serine/Threonine-Protein Kinase PLK1 (PLK1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Serine/threonine-protein kinase PLK1 (PLK1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Serine/threonine-protein kinase PLK1 (PLK1) Standard Curve.)
Background: Plk1 consists of 603 amino acids and is 66kDa. In addition to the N-terminus kinase domain, there are two conserved polo-box regions of 30 amino acids at the C-terminus. Plk1 is an early trigger for G2/M transition. Plk1 supports the functional maturation of the centrosome in late G2/early prophase and establishment of the bipolar spindle. Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Plk phosphorylates and activates components of the APC. The APC, which is activated by Fizzy-Cdc20 family proteins, is a cell cycle ubiquitin-protein ligase (E3) that degrades mitotic cyclins, chromosomal proteins that maintain cohesion of sister chromatids, and anaphase inhibitors.
NCBI and Uniprot Product Information
NCBI Description
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and may play a role in DNA replication during S phase. This gene is expressed in all embryonic tissues, but restricted to thymus and ovaries in adult tissues. Homozygous knockout mice were embryonic lethal, suggesting that this gene is important for early embryonic development. This gene is thought to be a potential oncogene because it is overexpressed in a variety of tumors and tumor cell lines. Depletion of this protein in cancer cells has been shown to inhibit cell proliferation and suppress oncogenic transformation; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]
Uniprot Description
PLK1: a kinase of the PLK family. Contains a polo-box domain (PBD), a specific phosphoserine or phosphothreonine binding domain. Substrates include BRCA2, Myt1, NudC, Cdc25C, cyclin B1, Nlp and other mitotic proteins. Inhibited by ATR. Plays a role in regulation of cytokinesis and coordinating M-phase events.
Protein type: EC 2.7.11.21; Kinase, protein; Other group; PLK family; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 7 F2|7 65.52 cM
Cellular Component: centrosome; chromatin; condensed nuclear chromosome, pericentric region; cytoplasm; kinetochore; microtubule cytoskeleton; midbody; nucleolus; nucleus; spindle; spindle microtubule; spindle midzone; spindle pole; synaptonemal complex
Molecular Function: microtubule binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity
Biological Process: centrosome organization and biogenesis; cytokinesis; cytokinesis after mitosis; establishment of protein localization; female meiosis chromosome segregation; G2/M transition DNA damage checkpoint; G2/M transition of mitotic cell cycle; homologous chromosome segregation; microtubule bundle formation; mitosis; mitotic cell cycle spindle assembly checkpoint; mitotic sister chromatid segregation; negative regulation of apoptosis; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; nuclear envelope disassembly; peptidyl-serine phosphorylation; polar body extrusion after meiotic divisions; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of proteolysis; positive regulation of ubiquitin-protein ligase activity; protein amino acid phosphorylation; protein destabilization; protein ubiquitination; regulation of mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; regulation of protein binding
Research Articles on PLK1
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Product Notes
The Mouse PLK1 plk1 (Catalog #AAA9717337) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717337 ELISA Kit recognizes Mouse PLK1. It is sometimes possible for the material contained within the vial of "Serine/Threonine-Protein Kinase PLK1 (PLK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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