Typical Testing Data/Standard Curve (for reference only)
Sheep Activin Receptor Like Kinase 1 ELISA Kit | ALK1 elisa kit
Sheep Activin Receptor Like Kinase 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ALK1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALK1 antibody and an ALK1-HRP conjugate. The assay sample and buffer are incubated together with ALK1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALK1 concentration since ALK1 from samples and ALK1-HRP conjugate compete for the anti-ALK1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALK1 from the sample, fewer sites are left to bind ALK1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALK1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2. [provided by RefSeq, Jul 2008]
Uniprot Description
ALK1: a kinase of the STKR family. A type I cell-surface receptor for the TGF-beta superfamily of ligands including Activin A. Highly expressed in human placenta and lung. Deficiency causes hemorrhagic telangiectasia type 2 also known as Rendu-Osler-Weber syndrome 2.
Protein type: EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; Membrane protein, integral; Kinase, protein; TKL group; STKR family; Type1 subfamily
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: cell surface; cell soma; integral to plasma membrane; dendrite
Molecular Function: transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; transforming growth factor beta receptor activity, type I; transforming growth factor beta binding; metal ion binding; activin binding; SMAD binding; protein kinase binding; ATP binding; transmembrane receptor protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: blood circulation; protein heterooligomerization; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; negative regulation of cell adhesion; regulation of blood vessel endothelial cell migration; signal transduction; protein amino acid phosphorylation; BMP signaling pathway; negative regulation of cell proliferation; positive regulation of endothelial cell differentiation; negative regulation of endothelial cell differentiation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; regulation of blood pressure; lymphangiogenesis; angiogenesis; negative regulation of cell migration; positive regulation of BMP signaling pathway; regulation of DNA replication; regulation of endothelial cell proliferation; in utero embryonic development; negative regulation of focal adhesion formation; negative regulation of blood vessel endothelial cell migration; blood vessel maturation; blood vessel endothelial cell proliferation during sprouting angiogenesis; positive regulation of angiogenesis; positive regulation of chondrocyte differentiation; negative regulation of endothelial cell proliferation; positive regulation of endothelial cell proliferation; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; wound healing, spreading of epidermal cells
Disease: Telangiectasia, Hereditary Hemorrhagic, Type 2
Research Articles on ALK1
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Product Notes
The Sheep ALK1 acvrl1 (Catalog #AAA755128) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA755128 ELISA Kit recognizes Sheep ALK1. It is sometimes possible for the material contained within the vial of "Activin Receptor Like Kinase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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