Typical Testing Data/Standard Curve (for reference only)
Canine Phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit gamma isoform (PIK3CG) ELISA Kit | PIK3CG elisa kit
Canine Phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit gamma isoform (PIK3CG) ELISA Kit
Reactivity
Canine
Synonyms
Phosphatidylinositol-4; 5-bisphosphate 3-kinase catalytic subunit gamma isoform (PIK3CG); Canine Phosphatidylinositol-4; 5-bisphosphate 3-kinase catalytic subunit gamma isoform (PIK3CG) ELISA Kit; PIK3CG elisa kit
Reactivity
Canine
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PIK3CG elisa kit
Intended Uses: This PIK3CG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PIK3CG. This ELISA kit for research use only!
Principle of the Assay: PIK3CG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PIK3CG antibody and an PIK3CG-HRP conjugate. The assay sample and buffer are incubated together with PIK3CG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PIK3CG concentration since PIK3CG from samples and PIK3CG-HRP conjugate compete for the anti-PIK3CG antibody binding site. Since the number of sites is limited, as more sites are occupied by PIK3CG from the sample, fewer sites are left to bind PIK3CG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIK3CG concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PIK3CG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PIK3CG antibody and an PIK3CG-HRP conjugate. The assay sample and buffer are incubated together with PIK3CG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PIK3CG concentration since PIK3CG from samples and PIK3CG-HRP conjugate compete for the anti-PIK3CG antibody binding site. Since the number of sites is limited, as more sites are occupied by PIK3CG from the sample, fewer sites are left to bind PIK3CG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIK3CG concentration in each sample is interpolated from this standard curve.
Product Categories/Family for PIK3CG elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
126,363 Da
NCBI Official Full Name
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
NCBI Official Symbol
PIK3CG
NCBI Protein Information
phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma; phosphoinositide-3-kinase, catalytic, gamma polypeptide
UniProt Protein Name
Uncharacterized protein
Protein Family
UniProt Gene Name
PIK3CG
UniProt Entry Name
H2PN59_PONAB
Similar Products
Product Notes
The Canine PIK3CG pik3cg (Catalog #AAA7216100) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7216100 ELISA Kit recognizes Canine PIK3CG. It is sometimes possible for the material contained within the vial of "Phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit gamma isoform (PIK3CG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
