Human cAMP dependent protein kinase type II Alpha regulatory subunit (PRKAR2A) ELISA Kit | PRKAR2A elisa kit

Human cAMP dependent protein kinase type II Alpha regulatory subunit (PRKAR2A) ELISA Kit

Cat. Num. AAA7204505

• Gene Names: PRKAR2A; PKR2; PRKAR2
• Reactivity: Human
• Synonyms: cAMP dependent protein kinase type II Alpha regulatory subunit (PRKAR2A); Human cAMP dependent protein kinase type II Alpha regulatory subunit (PRKAR2A) ELISA Kit; PRKAR2A elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRKAR2A elisa kit

Intended Uses: This PRKACB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PRKACB. This ELISA kit for research use only!

Principle of the Assay: PRKACB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PRKACB antibody and an PRKACB-HRP conjugate. The assay sample and buffer are incubated together with PRKACB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRKACB concentration since PRKACB from samples and PRKACB-HRP conjugate compete for the anti-PRKACB antibody binding site. Since the number of sites is limited, as more sites are occupied by PRKACB from the sample, fewer sites are left to bind PRKACB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRKACB concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PRKAR2A elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4758958
• NCBI GeneID: 5576
• NCBI Accession #: NP_004148.1
• NCBI GenBank Nucleotide #: NM_004157.2
• Molecular Weight: 45,518 Da
• NCBI Official Full Name: cAMP-dependent protein kinase type II-alpha regulatory subunit
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type II, alpha
• NCBI Official Symbol: PRKAR2A
• NCBI Official Synonym Symbols: PKR2; PRKAR2
• NCBI Protein Information: cAMP-dependent protein kinase type II-alpha regulatory subunit; protein kinase A, RII-alpha subunit; cAMP-dependent protein kinase regulatory subunit RII alpha
• UniProt Protein Name: cAMP-dependent protein kinase type II-alpha regulatory subunit
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKAR2A
• UniProt Synonym Gene Names: PKR2; PRKAR2
• UniProt Entry Name: KAP2_HUMAN

NCBI Description

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]

Uniprot Description

PKAR2A: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 3p21.3-p21.2

Cellular Component: centrosome; focal adhesion; membrane; cytoplasm; plasma membrane; cytosol; cAMP-dependent protein kinase complex; AMP-activated protein kinase complex

Molecular Function: protein binding; cAMP-dependent protein kinase inhibitor activity; ubiquitin protein ligase binding; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; signal transduction; phospholipase C activation; energy reserve metabolic process; innate immune response; renal water homeostasis; blood coagulation; regulation of insulin secretion; transmembrane transport