Mouse Sclerostin Domain Containing Protein 1 (SOSTDC1) ELISA Kit | SOSTDC1 elisa kit

Mouse Sclerostin Domain Containing Protein 1 (SOSTDC1) ELISA Kit

Cat. Num. AAA454879

• Gene Names: Sostdc1; Wise; Sostl; USAG-1; ectodin; 0610006G05Rik
• Reactivity: Mouse
• Synonyms: Sclerostin Domain Containing Protein 1 (SOSTDC1); Mouse Sclerostin Domain Containing Protein 1 (SOSTDC1) ELISA Kit; SOSTDC1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of SOSTDC1. No significant cross-reactivity or interference between SOSTDC1 and analogues was observed.
• Sequence Length: 206

• Samples: Mouse serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.068ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SOSTDC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SOSTDC1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOSTDC1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SOSTDC1 in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SOSTDC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SOSTDC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SOSTDC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SOSTDC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13384674
• NCBI GeneID: 66042
• NCBI Accession #: NP_079588.1
• NCBI GenBank Nucleotide #: NM_025312.3
• UniProt Accession #: Q9CQN4; Q8CF09
• Molecular Weight: 23,174 Da
• NCBI Official Full Name: sclerostin domain-containing protein 1
• NCBI Official Synonym Full Names: sclerostin domain containing 1
• NCBI Official Symbol: Sostdc1
• NCBI Official Synonym Symbols: Wise; Sostl; USAG-1; ectodin; 0610006G05Rik
• NCBI Protein Information: sclerostin domain-containing protein 1
• UniProt Protein Name: Sclerostin domain-containing protein 1
• Protein Family: Sclerostin domain-containing protein
• UniProt Gene Name: Sostdc1
• UniProt Synonym Gene Names: Sostl; Usag1; Ectodin; USAG-1

Uniprot Description

May be involved in the onset of endometrial receptivity for implantation/sensitization for the decidual cell reaction. Enhances Wnt signaling and inhibits TGF-beta signaling (). Directly antagonizes activity of BMP2, BMP4, BMP6 and BMP7 in a dose-dependent manner.