Rabbit MCM2 Polyclonal Antibody | anti-MCM2 antibody

MCM2 Rabbit Polyclonal

Cat. Num. AAA7601289

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: MCM2; Polyclonal Antibody; MCM2 Rabbit Polyclonal; anti-MCM2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 890

• Applicable Applications for anti-MCM2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: Minichromosome maintenance complex component 2
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human placenta tissue slide using MBS7601289 (MCM2 Antibody) at dilution of 1:50)

Western Blot (WB)

(K-562 cells were subjected to SDS PAGE followed by western blot with MBS7601289 (MCM2 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 3912
• UniProt Accession #: P29469; D6VPX7
• Molecular Weight: 98,780 Da
• NCBI Official Full Name: MCM2
• UniProt Protein Name: DNA replication licensing factor MCM2
• Protein Family: DNA replication licensing factor
• UniProt Gene Name: MCM2
• UniProt Entry Name: MCM2_YEAST

Uniprot Description

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity; specifically the MCM2-MCM5 association is proposed to be reversible and to mediate a open ring conformation which may facilitate DNA loading. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Necessary for cell growth.