Human Sclerostin Domain Containing Protein 1 (SOSTDC1) RTU ELISA Kit | SOSTDC1 rtu elisa kit

Human Sclerostin Domain Containing Protein 1 (SOSTDC1) ELISA Kit

Cat. Num. AAA4502595

• Gene Names: SOSTDC1; DAND7; USAG1; CDA019; ECTODIN
• Reactivity: Human
• Synonyms: Sclerostin Domain Containing Protein 1 (SOSTDC1); Human Sclerostin Domain Containing Protein 1 (SOSTDC1) ELISA Kit; CDA019; USAG1; Ectodin; Ectodermal BMP inhibitor; Uterine sensitization-associated gene 1 protein; SOSTDC1 rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SOSTDC1. No significant cross-reactivity or interference between SOSTDC1 and analogues was observed.
• Sequence Length: 206

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SOSTDC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SOSTDC1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOSTDC1 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SOSTDC1 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SOSTDC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SOSTDC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SOSTDC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SOSTDC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 23943785
• NCBI GeneID: 25928
• NCBI Accession #: NP_056279.1
• NCBI GenBank Nucleotide #: NM_015464.2
• UniProt Accession #: Q6X4U4; Q96HJ7; Q9Y3U3; A8MUA6
• Molecular Weight: 26,260 Da
• NCBI Official Full Name: sclerostin domain-containing protein 1
• NCBI Official Synonym Full Names: sclerostin domain containing 1
• NCBI Official Symbol: SOSTDC1
• NCBI Official Synonym Symbols: DAND7; USAG1; CDA019; ECTODIN
• NCBI Protein Information: sclerostin domain-containing protein 1
• UniProt Protein Name: Sclerostin domain-containing protein 1
• Protein Family: Sclerostin domain-containing protein
• UniProt Gene Name: SOSTDC1
• UniProt Synonym Gene Names: USAG1; Ectodin; USAG-1

NCBI Description

This gene is a member of the sclerostin family and encodes an N-glycosylated, secreted protein with a C-terminal cystine knot-like domain. This protein functions as a bone morphogenetic protein (BMP) antagonist. Specifically, it directly associates with BMPs, prohibiting them from binding their receptors, thereby regulating BMP signaling during cellular proliferation, differentiation, and programmed cell death. [provided by RefSeq, Jul 2008]

Uniprot Description

May be involved in the onset of endometrial receptivity for implantation/sensitization for the decidual cell reaction Enhances Wnt signaling and inhibits TGF-beta signaling (). Directly antagonizes activity of BMP2, BMP4, BMP6 and BMP7 in a dose-dependent manner.