Mouse Regulator Of G Protein Signaling 7 (RGS7) ELISA Kit | RGS7 elisa kit

Mouse Regulator Of G Protein Signaling 7 (RGS7) ELISA Kit

Cat. Num. AAA2705209

• Reactivity: Mouse
• Synonyms: Regulator Of G Protein Signaling 7 (RGS7); Mouse Regulator Of G Protein Signaling 7 (RGS7) ELISA Kit; RGS7 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Regulator Of G Protein Signaling 7 (RGS7).; No significant cross-reactivity or interference between Regulator Of G Protein Signaling 7 (RGS7) and analogues was observed.
• Sequence Length: 477

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulator Of G Protein Signaling 7 (RGS7) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulator Of G Protein Signaling 7 (RGS7) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RGS7 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulator Of G Protein Signaling 7 (RGS7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regulator Of G Protein Signaling 7 (RGS7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulator Of G Protein Signaling 7 (RGS7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regulator Of G Protein Signaling 7 (RGS7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9507047
• NCBI GeneID: 54296
• NCBI Accession #: NP_062216.1
• NCBI GenBank Nucleotide #: NM_019343.1
• UniProt Accession #: P49803; Q9R0R0
• Molecular Weight: 55,691 Da
• NCBI Official Full Name: regulator of G-protein signaling 7
• NCBI Official Synonym Full Names: regulator of G-protein signaling 7
• NCBI Official Symbol: Rgs7
• NCBI Protein Information: regulator of G-protein signaling 7
• UniProt Protein Name: Regulator of G-protein signaling 7
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: Rgs7
• UniProt Synonym Gene Names: RGS7

NCBI Description

acts as a GTPase activating protein; regulates activation and deactivation kinetics of G-protein coupled inward rectifier K+ channel; binds Galphao; Galphai3, and Galphaz [RGD, Feb 2006]

Uniprot Description

RGS7: inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(o)-alpha is specifically enhanced by the RGS6/GNG5 dimer. May play a role in synaptic vesicle exocytosis. May play important role in the rapid regulation of neuronal excitability and the cellular responses to short-lived stimulations. Heterodimer with GNG5. Interacts with RGS7BP, leading to regulate the subcellular location of the heterodimer formed with Gbeta5 (By similarity). Interacts with 14-3-3 protein Tau and SNAP25BP. Four alternatively spliced isoforms have been described.

Protein type: GAP; GAP, RGS

Chromosomal Location of Human Ortholog: 13q24-q25

Cellular Component: cytosol; heterotrimeric G-protein complex; nucleus; plasma membrane; protein complex

Molecular Function: G-protein beta-subunit binding; GTPase activator activity; protein binding; signal transducer activity

Biological Process: G-protein coupled receptor signaling pathway; negative regulation of signal transduction; positive regulation of GTPase activity; regulation of G-protein coupled receptor protein signaling pathway