Human Regulator Of G Protein Signaling 5 (RGS5) ELISA Kit | RGS5 elisa kit

Human Regulator Of G Protein Signaling 5 (RGS5) ELISA Kit

Cat. Num. AAA2705205

• Gene Names: RGS5; MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129
• Reactivity: Human
• Synonyms: Regulator Of G Protein Signaling 5 (RGS5); Human Regulator Of G Protein Signaling 5 (RGS5) ELISA Kit; RGS5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Regulator Of G Protein Signaling 5 (RGS5).; No significant cross-reactivity or interference between Regulator Of G Protein Signaling 5 (RGS5) and analogues was observed.
• Sequence Length: 185

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.125ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulator Of G Protein Signaling 5 (RGS5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulator Of G Protein Signaling 5 (RGS5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RGS5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulator Of G Protein Signaling 5 (RGS5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regulator Of G Protein Signaling 5 (RGS5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulator Of G Protein Signaling 5 (RGS5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regulator Of G Protein Signaling 5 (RGS5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 20988750
• NCBI GeneID: 8490
• Molecular Weight: 21,402 Da
• NCBI Official Full Name: Regulator of G-protein signaling 5
• NCBI Official Synonym Full Names: regulator of G protein signaling 5
• NCBI Official Symbol: RGS5
• NCBI Official Synonym Symbols: MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129
• NCBI Protein Information: regulator of G-protein signaling 5
• UniProt Protein Name: Regulator of G-protein signaling 5
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS5
• UniProt Synonym Gene Names: RGS5

NCBI Description

This gene encodes a member of the regulators of G protein signaling (RGS) family. The RGS proteins are signal transduction molecules which are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. This gene is a hypoxia-inducible factor-1 dependent, hypoxia-induced gene which is involved in the induction of endothelial apoptosis. This gene is also one of three genes on chromosome 1q contributing to elevated blood pressure. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Dec 2011]

Uniprot Description

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha ().