Mouse Reelin (RELN) ELISA Kit | RELN elisa kit

Mouse Reelin (RELN) Mini Samples ELISA Kit

Cat. Num. AAA2708390

• Gene Names: Reln; rl; reeler
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Reelin (RELN); Mouse Reelin (RELN) Mini Samples ELISA Kit; Reelin; RL; RELN elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Reelin (RELN).; No significant cross-reactivity or interference between Mini Samples Reelin (RELN) and analogues was observed.
• Sequence Length: 3459

• Applicable Applications for RELN elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, cell lysates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.055ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Reelin (RELN) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Reelin (RELN) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Reelin (RELN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Reelin (RELN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Reelin (RELN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Reelin (RELN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 882939033
• NCBI GeneID: 19699
• NCBI Accession #: NP_001297393.1
• NCBI GenBank Nucleotide #: NP_001297393.1
• UniProt Accession #: Q60841
• NCBI Official Full Name: reelin isoform 2
• NCBI Official Synonym Full Names: reelin
• NCBI Official Symbol: Reln
• NCBI Official Synonym Symbols: rl; reeler
• NCBI Protein Information: reelin
• UniProt Protein Name: Reelin
• Protein Family: Reelin
• UniProt Gene Name: Reln
• UniProt Synonym Gene Names: Rl

Uniprot Description

Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation.