Rabbit anti-Human, Mouse GPER Polyclonal Antibody | anti-GPER antibody

GPER, CT (GPER, CEPR, CMKRL2, DRY12, GPR30, G-protein coupled estrogen receptor 1, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1, G-protein coupled receptor 30, GPCR-BR, IL8-related receptor DRY12, Lymphocyte-deriv

Cat. Num. AAA6303523

• Gene Names: GPER1; mER; CEPR; GPER; DRY12; FEG-1; GPR30; LERGU; LyGPR; CMKRL2; LERGU2; GPCR-Br
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPER; Polyclonal Antibody; CT (GPER; CEPR; CMKRL2; DRY12; GPR30; G-protein coupled estrogen receptor 1; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1; G-protein coupled receptor 30; GPCR-BR; IL8-related receptor DRY12; Lymphocyte-deriv; Lymphocyte-derived G-protein coupled receptor; Membrane estrogen receptor; anti-GPER antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-GPER antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GPER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-375 amino acids from the C-terminal region of human GPER.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GPER Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the GPER antibody detected the GPER protein (arrow).)

Western Blot (WB)

(GPER Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GPER antibody detected the GPER protein (arrow).)

Related Product Information for anti-GPER antibody

This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq].

Product Categories/Family for anti-GPER antibody

Antibodies; Receptors; Estrogen

References

Patel, V.H., et al. Int. J. Mol. Med. 26(2):193-199(2010). Ignatov, A., et al. Breast Cancer Res. Treat. 123(1):87-96(2010). Li, Y., et al. Mol. Endocrinol. 24(5):969-980(2010). Kang, L., et al. Mol. Endocrinol. 24(4):709-721(2010). Ariazi, E.A., et al. Cancer Res. 70(3):1184-1194(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 148228791
• NCBI GeneID: 2852
• NCBI Accession #: NP_001091671.1; NP_001496.1
• NCBI GenBank Nucleotide #: NM_001098201.2
• UniProt Accession #: Q99527; O00143; O43494; Q13631; Q6FHL1; Q96F42; Q99981; A8K6C5; B5BUJ1
• Molecular Weight: 42,248 Da
• NCBI Official Full Name: G-protein coupled estrogen receptor 1
• NCBI Official Synonym Full Names: G protein-coupled estrogen receptor 1
• NCBI Official Symbol: GPER1
• NCBI Official Synonym Symbols: mER; CEPR; GPER; DRY12; FEG-1; GPR30; LERGU; LyGPR; CMKRL2; LERGU2; GPCR-Br
• NCBI Protein Information: G-protein coupled estrogen receptor 1
• UniProt Protein Name: G-protein coupled estrogen receptor 1
• UniProt Gene Name: GPER1
• UniProt Synonym Gene Names: CEPR; CMKRL2; DRY12; GPER; GPR30; FEG-1; LYGPR; mER
• UniProt Entry Name: GPER1_HUMAN

NCBI Description

This gene encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum and a member of the G-protein coupled receptor 1 family. This receptor binds estrogen and activates multiple downstream signaling pathways, leading to stimulation of adenylate cyclase and an increase in cyclic AMP levels, while also promoting intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. This receptor has been shown to play a role in diverse biological processes, including bone and nervous system development, metabolism, cognition, male fertility and uterine function. [provided by RefSeq, Aug 2017]

Uniprot Description

GPER1: Receptor for estrogen. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: Golgi apparatus; integral to plasma membrane; early endosome; dendrite; nuclear envelope; trans-Golgi network; recycling endosome; perinuclear region of cytoplasm; cytoplasm; keratin filament; intracellular; dendritic shaft; endoplasmic reticulum membrane; cytoplasmic vesicle membrane; endoplasmic reticulum; postsynaptic density; Golgi membrane; presynaptic membrane; axon; mitochondrial membrane; presynaptic active zone; plasma membrane; nerve terminal; nucleus; cell junction

Molecular Function: G-protein coupled receptor activity; protein binding; estrogen receptor activity; chromatin binding; mineralocorticoid receptor activity; steroid binding

Biological Process: positive regulation of apoptosis; generation of action potential; positive regulation of caspase activity; nuclear fragmentation during apoptosis; negative regulation of DNA metabolic process; positive regulation of vasodilation; positive regulation of neurotransmitter secretion; negative regulation of leukocyte activation; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; inflammatory response; steroid hormone receptor signaling pathway; positive regulation of neurogenesis; cytosolic calcium ion homeostasis; negative regulation of lipid biosynthetic process; positive regulation of insulin secretion; positive regulation of inositol trisphosphate biosynthetic process; cell cycle; negative regulation of fat cell differentiation; apoptotic chromosome condensation; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; mineralocorticoid receptor signaling pathway; negative regulation of inflammatory response; positive regulation of G-protein coupled receptor protein signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cell migration