Mouse Fibrinogen Alpha Chain (FGA) ELISA Kit | FGA elisa kit

Mouse Fibrinogen Alpha Chain (FGA) Mini Samples ELISA Kit

Cat. Num. AAA2708346

• Gene Names: Fga; Fib
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Fibrinogen Alpha Chain (FGA); Mouse Fibrinogen Alpha Chain (FGA) Mini Samples ELISA Kit; Fibrinogen Alpha Chain; Fib2; FG-A; FGA; Fibrinopeptide A; FGA elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Fibrinogen Alpha Chain (FGA).; No significant cross-reactivity or interference between Mini Samples Fibrinogen Alpha Chain (FGA) and analogues was observed.
• Sequence Length: 789

• Applicable Applications for FGA elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Plasma
• Detection Range: 0.625-40ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.251ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Fibrinogen Alpha Chain (FGA) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Fibrinogen Alpha Chain (FGA) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Fibrinogen Alpha Chain (FGA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Fibrinogen Alpha Chain (FGA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Fibrinogen Alpha Chain (FGA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Fibrinogen Alpha Chain (FGA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 167555029
• NCBI GeneID: 14161
• NCBI Accession #: NP_001104518.1
• NCBI GenBank Nucleotide #: NP_001104518.1
• UniProt Accession #: E9PV24
• NCBI Official Full Name: fibrinogen alpha chain isoform 1 preproprotein
• NCBI Official Synonym Full Names: fibrinogen alpha chain
• NCBI Official Symbol: Fga
• NCBI Official Synonym Symbols: Fib
• NCBI Protein Information: fibrinogen alpha chain
• UniProt Protein Name: Fibrinogen alpha chain
• Protein Family: Fibrinogen
• UniProt Gene Name: Fga

NCBI Description

This gene encodes a subunit of the coagulation factor fibrinogen, which is a component of the blood clot. The encoded protein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mice lacking the encoded protein display bleeding in the peritoneal cavity, skin and soft tissues around joints immediately after birth, and are predisposed to spontaneous fatal abdominal hemorrhage as they grow. Pregnant mice lacking the encoded protein succumb to uterine bleeding during gestation. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Nov 2015]

Uniprot Description

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots (PubMed:7649481). In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization (PubMed:11389004). Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood (PubMed:7649481). However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo (PubMed:10930441). Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway (PubMed:19332769). Maternal fibrinogen is essential for successful pregnancy (PubMed:7649481). Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage (PubMed:12629066). May also facilitate the immune response via both innate and T-cell mediated pathways (PubMed:23487423).