Rat Fractalkine ELISA Kit | CX3CL1 elisa kit

Rat Fractalkine PicoKine ELISA Kit

Cat. Num. AAA177370

• Gene Names: Cx3cl1; Cx3c; Scyd1
• Reactivity: Rat
• Synonyms: Fractalkine; Rat Fractalkine PicoKine ELISA Kit; chemokine (C-X3-C motif) ligand 1; A 152E5.2; AB030188; ABCD 3; ABCD3; C X3 C motif chemokine 1; C3Xkine; Chemokine (C X3 C motif) ligand 1; Chemokine C X3 C motif ligand 1; Chemokine CX3C Motif Ligand 1; CX3C membrane anchored chemokine; CXC3; CXC3C; FKN; Neurotactin; NTN; NTT; SCYD 1; SCYD1; CX3XL1; Small inducible cytokine D1; Small inducible cytokine subfamily D (Cys X3 Cys); member 1; Small inducible cytokine subfamily D (Cys-X3-Cys); member 1 (fractalkine; neurotactin); Small inducible cytokine subfamily D member 1; CX3CL1 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat Fractalkine
• Sequence Length: 393

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA), and tissue homogenates
• Assay Type: Sandwich
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Rat Fractalkine PicoKine ELISA Kit standard curve )

Related Product Information for CX3CL1 elisa kit

Principle of the assay: rat Fractalkine ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Fractalkine has been precoated onto 96-well plates. Standards (sf21, Q25-T334) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fractalkine is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Fractalkine amount of sample captured in plate.
Background: Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice).1, 2 CX3CL1 is produced as a long protein (with 373-amino acid inhumans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.2 CX3CL1 elicits its adhesiveand migratory functions by interacting with the chemokine receptor CX3CR1.3 Its gene is located on humanchromosome 16 along with some CC chemokines known as CCL17 and CCL22.2, 4 It can act as a mediator ofsmooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.5

NCBI and Uniprot Product Information

• NCBI GI #: 18266784
• NCBI GeneID: 89808
• UniProt Accession #: O55145
• Molecular Weight: 41,977 Da
• NCBI Official Full Name: Fractalkine
• NCBI Official Synonym Full Names: chemokine (C-X3-C motif) ligand 1
• NCBI Official Symbol: Cx3cl1
• NCBI Official Synonym Symbols: Cx3c; Scyd1
• NCBI Protein Information: fractalkine; neurotactin; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D, 1
• UniProt Protein Name: Fractalkine
• Protein Family: Fractalkine
• UniProt Gene Name: Cx3cl1
• UniProt Synonym Gene Names: Acc1; Fkn; Scyd1
• UniProt Entry Name: X3CL1_RAT

NCBI Description

chemokine that binds its receptor CX3CR1; induces chemotaxis and increased intracellular calcium levels in microglia [RGD, Feb 2006]

Uniprot Description

Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium.