Human RBP-4 ELISA Kit | RBP-4 elisa kit

RBP-4 Human ELISA Kit

Cat. Num. AAA355325

• Gene Names: Rbp4; Rbp-4
• Reactivity: Human
• Applications: ELISA
• Synonyms: RBP-4; RBP-4 Human ELISA Kit; Human RBP-4; RBP-4 elisa kit

• Reactivity: Human

• Applicable Applications for RBP-4 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of RBP4 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
• Assay Type: Sandwich
• Detection Range: 500pg/ml- 10,000pg/ml.
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RBP-4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. The purified anti-E-selectinantibody was pre-coated onto 96-well plates. And the HRP conjugated anti-E-selectin antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A&B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the E- selectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of E- selectin can be calculated.

Background: E-selectin, also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), is a cell adhesion molecule expressed only on endothelial cells activated by cytokines. The ELAM gene is present in single copy in the human genome and contains 14 exons spanning about 13 kb of DNA[1]. E-selectin mediates the adhesion of tumor cells to endothelial cells, by binding to E-selectin ligands expressed by neutrophils, monocytes, eosinophils, memory-effector T-like lymphocytes, natural killer cells or cancer cells.

NCBI and Uniprot Product Information

• NCBI GI #: 226958688
• NCBI GeneID: 19662
• NCBI Accession #: NP_001152959.1
• NCBI GenBank Nucleotide #: NM_001159487.1
• UniProt Accession #: Q00724; P70357; Q566I5
• Molecular Weight: 23,206 Da
• NCBI Official Full Name: retinol-binding protein 4 isoform 1
• NCBI Official Synonym Full Names: retinol binding protein 4, plasma
• NCBI Official Symbol: Rbp4
• NCBI Official Synonym Symbols: Rbp-4
• NCBI Protein Information: retinol-binding protein 4; RBP; PRBP; plasma retinol-binding protein; retinol binding protein 4, cellular
• UniProt Protein Name: Retinol-binding protein 4
• UniProt Gene Name: Rbp4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_MOUSE

Uniprot Description

Function: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.

Subcellular location: Secreted.

Sequence similarities: Belongs to the calycin superfamily. Lipocalin family.