Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein delta homolog 2 (DLK2) Standard Curve.)
Mouse Protein delta Homolog 2 (DLK2) ELISA Kit | DLK2 elisa kit
Mouse Protein delta Homolog 2 (DLK2) ELISA Kit
Gene Names
Dlk2; DLK-2; Egfl9; AI413481
Reactivity
Mouse
Synonyms
Protein delta Homolog 2 (DLK2); Mouse Protein delta Homolog 2 (DLK2) ELISA Kit; DLK2; EGFL9; MGC111055; MGC2487; EGF-like-domain; multiple 9; DLK2 elisa kit
Reactivity
Mouse
Specificity
Mouse Protein delta homolog 2 (DLK2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DLK2. No significant cross-reactivity or interference between Mouse DLK2 and analogues was observed.
Sequence Length
382
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein delta homolog 2 (DLK2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein delta homolog 2 (DLK2) Standard Curve.)
Related Product Information for DLK2 elisa kit
Description: This Mouse Protein delta homolog 2 (DLK2) ELISA Kit employs a two-site sandwich ELISA to quantitate DLK2 in samples. An antibody specific for DLK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The Dlk1 gene appears to function as a regulator of adipogenesis. Adult Dlk1-deficient mice are obese, but adipose tissue still develops in transgenic mice overexpressing an Fc-dlk1 fusion protein, and neither type of genetically modified mice displays serious abnormalities. EGFL9, encoding for a protein whose structural features are virtually identical to those of dlk1, suggesting it may function in a similar way. As dlk1 does, the protein encoded by EGFL9/Dlk2 affects adipogenesis of 3T3-L1 preadipocytes and mesenchymal C3H10T1/2 cells; however, it does so in an opposite way to that of dlk1. In addition, expression levels of both genes appear to be inversely correlated in both cell lines. Moreover, enforced changes in the expression of one gene affect the expression levels of the other.
Background: The Dlk1 gene appears to function as a regulator of adipogenesis. Adult Dlk1-deficient mice are obese, but adipose tissue still develops in transgenic mice overexpressing an Fc-dlk1 fusion protein, and neither type of genetically modified mice displays serious abnormalities. EGFL9, encoding for a protein whose structural features are virtually identical to those of dlk1, suggesting it may function in a similar way. As dlk1 does, the protein encoded by EGFL9/Dlk2 affects adipogenesis of 3T3-L1 preadipocytes and mesenchymal C3H10T1/2 cells; however, it does so in an opposite way to that of dlk1. In addition, expression levels of both genes appear to be inversely correlated in both cell lines. Moreover, enforced changes in the expression of one gene affect the expression levels of the other.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein delta homolog 2
NCBI Official Synonym Full Names
delta like non-canonical Notch ligand 2
NCBI Official Symbol
Dlk2
NCBI Official Synonym Symbols
DLK-2; Egfl9; AI413481
NCBI Protein Information
protein delta homolog 2
UniProt Protein Name
Protein delta homolog 2
Protein Family
UniProt Gene Name
Dlk2
UniProt Synonym Gene Names
Egfl9; DLK-2; EGF-like protein 9
UniProt Entry Name
DLK2_MOUSE
Research Articles on DLK2
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Product Notes
The Mouse DLK2 dlk2 (Catalog #AAA9717856) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717856 ELISA Kit recognizes Mouse DLK2. It is sometimes possible for the material contained within the vial of "Protein delta Homolog 2 (DLK2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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