Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Delta-like protein 3 (DLL3) Standard Curve.)
Rat Delta-Like Protein 3 (DLL3) ELISA Kit | DLL3 elisa kit
Rat Delta-Like Protein 3 (DLL3) ELISA Kit
Reactivity
Rat
Synonyms
Delta-Like Protein 3 (DLL3); Rat Delta-Like Protein 3 (DLL3) ELISA Kit; DLL3; SCDO1; delta-like 3 protein; DLL3 elisa kit
Reactivity
Rat
Specificity
Rat Delta-like protein 3 (DLL3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLL3. No significant cross-reactivity or interference between Rat DLL3 and analogues was observed.
Sequence Length
589
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Delta-like protein 3 (DLL3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Delta-like protein 3 (DLL3) Standard Curve.)
Related Product Information for DLL3 elisa kit
Description: This Rat Delta-like protein 3 (DLL3) ELISA Kit employs a two-site sandwich ELISA to quantitate DLL3 in samples. An antibody specific for DLL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DLL3 encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. In humans, the fact that mutations in genes required for oscillation, such as DLL3, result in abnormal segmentation of the vertebral column suggests that the segmentation clock also acts during human embryonic development. Disruption of the Notch pathway occurs in Alagille syndrome, a disorder that has vertebral abnormalities, i.e., 'butterfly vertebrae,' as a feature in about two-thirds of patients.
Background: DLL3 encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. In humans, the fact that mutations in genes required for oscillation, such as DLL3, result in abnormal segmentation of the vertebral column suggests that the segmentation clock also acts during human embryonic development. Disruption of the Notch pathway occurs in Alagille syndrome, a disorder that has vertebral abnormalities, i.e., 'butterfly vertebrae,' as a feature in about two-thirds of patients.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
delta-like protein 3
NCBI Official Synonym Full Names
delta like canonical Notch ligand 3
NCBI Official Symbol
Dll3
NCBI Protein Information
delta-like protein 3
UniProt Protein Name
Delta-like protein 3
Protein Family
UniProt Gene Name
Dll3
UniProt Synonym Gene Names
Delta3
UniProt Entry Name
DLL3_RAT
NCBI Description
homolog of the Notch Delta ligand; member of the delta/serrate/jagged family; may play a role in cell fate decisions during hematopoiesis [RGD, Feb 2006]
Uniprot Description
Function: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm
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Product Notes
The Rat DLL3 dll3 (Catalog #AAA9712415) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712415 ELISA Kit recognizes Rat DLL3. It is sometimes possible for the material contained within the vial of "Delta-Like Protein 3 (DLL3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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