Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein delta homolog 1 (DLK1) Standard Curve.)
Human Protein delta Homolog 1 (DLK1) ELISA Kit | DLK1 elisa kit
Human Protein delta Homolog 1 (DLK1) ELISA Kit
Gene Names
DLK1; DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1
Reactivity
Human
Synonyms
Protein delta Homolog 1 (DLK1); Human Protein delta Homolog 1 (DLK1) ELISA Kit; DLK1; DLK; FA1; PREF1; Pref-1; ZOG; pG2; delta-like 1 homolog; delta-like homolog; fetal antigen 1; preadipocyte factor 1; secredeltin; DLK1 elisa kit
Reactivity
Human
Specificity
Human Protein delta homolog 1 (DLK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DLK1. No significant cross-reactivity or interference between Human DLK1 and analogues was observed.
Sequence Length
383
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein delta homolog 1 (DLK1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein delta homolog 1 (DLK1) Standard Curve.)
Related Product Information for DLK1 elisa kit
Description: This Human Protein delta homolog 1 (DLK1) ELISA Kit employs a two-site sandwich ELISA to quantitate DLK1 in samples. An antibody specific for DLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DLK1 encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass.
Background: DLK1 encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein delta homolog 1 isoform 1 preproprotein
NCBI Official Synonym Full Names
delta like non-canonical Notch ligand 1
NCBI Official Symbol
DLK1
NCBI Official Synonym Symbols
DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1
NCBI Protein Information
protein delta homolog 1
UniProt Protein Name
Protein delta homolog 1
Protein Family
UniProt Gene Name
DLK1
UniProt Synonym Gene Names
DLK; DLK-1; FA1
UniProt Entry Name
DLK1_HUMAN
NCBI Description
This gene encodes a transmembrane protein that contains multiple epidermal growth factor repeats that functions as a regulator of cell growth. The encoded protein is involved in the differentiation of several cell types including adipocytes. This gene is located in a region of chromosome 14 frequently showing unparental disomy, and is imprinted and expressed from the paternal allele. A single nucleotide variant in this gene is associated with child and adolescent obesity and shows polar overdominance, where heterozygotes carrying an active paternal allele express the phenotype, while mutant homozygotes are normal. [provided by RefSeq, Nov 2015]
Research Articles on DLK1
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Product Notes
The Human DLK1 dlk1 (Catalog #AAA9715683) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715683 ELISA Kit recognizes Human DLK1. It is sometimes possible for the material contained within the vial of "Protein delta Homolog 1 (DLK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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