Western Blot (WB)
(ZP4 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ZP4 antibody detected the ZP4 protein (arrow).)
Rabbit anti-Human ZP4 Polyclonal Antibody | anti-ZP4 antibody
ZP4 Antibody (C-term)
Western Blot (WB)
(ZP4 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ZP4 antibody detected the ZP4 protein (arrow).)
Western Blot (WB)
(ZP4 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ZP4 antibody detected the ZP4 protein (arrow).)
Immunohistochemistry (IHC)
(ZP4 Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZP4 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ZP4 Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZP4 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
surrounds the oocyte and early embryo. It is composed primarily of
three or four glycoproteins with various functions during
fertilization and preimplantation development. The nascent protein
contains a N-terminal signal peptide sequence, a conserved ZP
domain, a consensus furin cleavage site, and a C-terminal
transmembrane domain. It is hypothesized that furin cleavage
results in release of the mature protein from the plasma membrane
for subsequent incorporation into the zona pellucida matrix.
However, the requirement for furin cleavage in this process remains
controversial based on mouse studies. Previously, this gene has
been referred to as ZP1 or ZPB and thought to have similar
functions as mouse Zp1. However, a human gene with higher
similarity and chromosomal synteny to mouse Zp1 has been assigned
the symbol ZP1 and this gene has been assigned the symbol ZP4.
McCauley, J.L., et al. Genes Immun. 10(7):624-630(2009)
Nakano, M., et al. Proc. Natl. Acad. Sci. U.S.A. 106(31):12838-12842(2009)
Choudhury, S., et al. J. Reprod. Immunol. 79(2):137-147(2009)
Chiu, P.C., et al. Biol. Reprod. 79(5):869-877(2008)
NCBI and Uniprot Product Information
NCBI Description
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. [provided by RefSeq, Jul 2008]
Uniprot Description
ZP4: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor. Belongs to the ZP domain family. ZPB subfamily.
Protein type: Membrane protein, integral; Extracellular matrix
Chromosomal Location of Human Ortholog: 1q43
Cellular Component: proteinaceous extracellular matrix; plasma membrane; integral to membrane; extracellular region
Molecular Function: acrosin binding; signal transducer activity
Biological Process: binding of sperm to zona pellucida; positive regulation of humoral immune response; single fertilization; positive regulation of protein kinase activity; positive regulation of T cell proliferation; multicellular organism reproduction
Research Articles on ZP4
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Product Notes
The ZP4 zp4 (Catalog #AAA9212766) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 445-474. The ZP4 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZP4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the ZP4 zp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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