Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prolactin (PRL) Standard Curve.)
Mouse Prolactin (PRL) ELISA Kit | PRL elisa kit
Mouse Prolactin (PRL) ELISA Kit
Gene Names
Prl; Gha1; Prl1a1; AV290867
Reactivity
Mouse
Synonyms
Prolactin (PRL); Mouse Prolactin (PRL) ELISA Kit; PRL; LTH; Luteotropic Hormone; PRL elisa kit
Reactivity
Mouse
Specificity
Mouse Prolactin (PRL) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRL. No significant cross-reactivity or interference between Mouse PRL and analogues was observed.
Sequence Length
226
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prolactin (PRL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prolactin (PRL) Standard Curve.)
Related Product Information for PRL elisa kit
Description: This Mouse Prolactin (PRL) ELISA Kit employs a two-site sandwich ELISA to quantitate PRL in samples. An antibody specific for PRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Luteotropic hormone is a peptide hormone discovered by Dr. Henry Friesen, primarily associated with lactation. In breastfeeding, the act of an infant suckling the nipple stimulates the production of prolactin, which fills the breast with milk via a process called lactogenesis, in preparation for the next feed. Oxytocin, another hormone, is also released, which triggers milk let-down.Prolactin (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of prolactin, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. Prolactin (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female.
Background: Luteotropic hormone is a peptide hormone discovered by Dr. Henry Friesen, primarily associated with lactation. In breastfeeding, the act of an infant suckling the nipple stimulates the production of prolactin, which fills the breast with milk via a process called lactogenesis, in preparation for the next feed. Oxytocin, another hormone, is also released, which triggers milk let-down.Prolactin (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of prolactin, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. Prolactin (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Prolactin
NCBI Official Synonym Full Names
prolactin
NCBI Official Symbol
Prl
NCBI Official Synonym Symbols
Gha1; Prl1a1; AV290867
NCBI Protein Information
prolactin
UniProt Protein Name
Prolactin
Protein Family
UniProt Gene Name
Prl
UniProt Synonym Gene Names
PRL
UniProt Entry Name
PRL_MOUSE
Uniprot Description
prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.
Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Cytokine; Secreted
Cellular Component: extracellular region; secretory granule
Molecular Function: prolactin receptor binding; hormone activity
Biological Process: lactation; regulation of JAK-STAT cascade; mammary gland development; maternal behavior; positive regulation of cell proliferation; positive regulation of JAK-STAT cascade; regulation of ossification; positive regulation of epithelial cell proliferation; STAT protein nuclear translocation
Research Articles on PRL
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Product Notes
The Mouse PRL prl (Catalog #AAA9717272) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717272 ELISA Kit recognizes Mouse PRL. It is sometimes possible for the material contained within the vial of "Prolactin (PRL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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