Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Pulmonary surfactant-associated protein A (SP-A) Standard Curve.)
Rat Pulmonary Surfactant-Associated Protein A (SP-A) ELISA Kit | SP-A elisa kit
Rat Pulmonary Surfactant-Associated Protein A (SP-A) ELISA Kit
Reactivity
Rat
Synonyms
Pulmonary Surfactant-Associated Protein A (SP-A); Rat Pulmonary Surfactant-Associated Protein A (SP-A) ELISA Kit; SFTPA1; AC068139.6; MGC133365; PSAP; PSPA; SFTP1; pulmonary surfactant apoprotein; surfactant; pulmonary-associated protein A1; pulmonary-associated protein A1B; SP-A elisa kit
Reactivity
Rat
Specificity
Rat Pulmonary surfactant-associated protein A (SP-A) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SFTPA1. No significant cross-reactivity or interference between Rat SFTPA1 and analogues was observed.
Sequence Length
247
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Pulmonary surfactant-associated protein A (SP-A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Pulmonary surfactant-associated protein A (SP-A) Standard Curve.)
Related Product Information for SP-A elisa kit
Description: This Rat Pulmonary surfactant-associated protein A (SP-A) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTPA1 in samples. An antibody specific for SFTPA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFTPA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SP-A (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of SP-A, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. SP-A (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female. Release of SP-A is controlled by a complex neuroendocrine reflex initiated by a tactile stimulus and regulated by hypothalamic releasing and inhibition. Genetic variations in SFTPA1 are associated with respiratory distress syndrome in premature infants (RDS); also known as RDS in prematurity. RDS in the newborn is the main cause of mortality and morbidity in premature infants.
Background: SP-A (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of SP-A, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. SP-A (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female. Release of SP-A is controlled by a complex neuroendocrine reflex initiated by a tactile stimulus and regulated by hypothalamic releasing and inhibition. Genetic variations in SFTPA1 are associated with respiratory distress syndrome in premature infants (RDS); also known as RDS in prematurity. RDS in the newborn is the main cause of mortality and morbidity in premature infants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pulmonary surfactant-associated protein A
NCBI Official Symbol
SFTPA1
NCBI Protein Information
pulmonary surfactant-associated protein A
UniProt Protein Name
Pulmonary surfactant-associated protein A
UniProt Gene Name
SFTPA1
UniProt Synonym Gene Names
SFTP1; SFTPA; PSAP; PSP-A; SP-A
UniProt Entry Name
SFTPA_RABIT
Uniprot Description
Function: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.
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Product Notes
The Rat SP-A sftpa1 (Catalog #AAA9711888) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711888 ELISA Kit recognizes Rat SP-A. It is sometimes possible for the material contained within the vial of "Pulmonary Surfactant-Associated Protein A (SP-A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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