Mouse Plet1 (Placenta-expressed transcript 1 protein) ELISA Kit | Plet1 elisa kit

Mouse Plet1 (Placenta-expressed transcript 1 protein) ELISA Kit

Cat. Num. AAA7606724

• Gene Names: PLET1; C11orf34
• Reactivity: Mouse
• Synonyms: Plet1 (Placenta-expressed transcript 1 protein); Mouse Plet1 (Placenta-expressed transcript 1 protein) ELISA Kit; Placenta-expressed transcript 1 protein; Plet1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of PLCgamma1. No significant cross-reactivity or interference between PLCgamma1 and analogues was observed.
• Sequence Length: 207

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLC gamma1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLC gamma1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Plet1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PLCgamma1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PLCgamma1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PLCgamma1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PLCgamma1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 222537734
• NCBI GeneID: 349633
• NCBI Accession #: NP_001138496.1
• NCBI GenBank Nucleotide #: NM_001145024.1
• UniProt Accession #: Q6UQ28; Q6UQ24; Q6UQ25; Q6UQ27
• Molecular Weight: 23,385 Da
• NCBI Official Full Name: placenta-expressed transcript 1 protein
• NCBI Official Synonym Full Names: placenta expressed transcript 1
• NCBI Official Symbol: PLET1
• NCBI Official Synonym Symbols: C11orf34
• NCBI Protein Information: placenta-expressed transcript 1 protein
• UniProt Protein Name: Placenta-expressed transcript 1 protein
• Protein Family: Placenta-expressed transcript 1 protein
• UniProt Gene Name: PLET1
• UniProt Synonym Gene Names: C11orf34

Uniprot Description

PLET1: May participate in the wound response during the healing process, and promote wound repair.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: apical plasma membrane; external side of plasma membrane; extracellular region; plasma membrane

Biological Process: C-terminal protein lipidation; negative regulation of cell-matrix adhesion; positive regulation of cell migration; wound healing, spreading of epidermal cells