Human Metastasis-suppressor KiSS-1 ELISA Kit | KISS1 elisa kit

Human Metastasis-suppressor KiSS-1 ELISA Kit

Cat. Num. AAA765519

• Gene Names: KISS1; HH13; KiSS-1
• Reactivity: Human
• Synonyms: Metastasis-suppressor KiSS-1; Human Metastasis-suppressor KiSS-1 ELISA Kit; KISS1 (Metastasis-suppressor KiSS-1)/Kisspeptin-1; KISS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kiss1. No significant cross-reactivity or interference between Kiss1 and analogues was observed.
• Sequence Length: 138

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kiss1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kiss1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KISS1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Kiss1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Kiss1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Kiss1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Kiss1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 116829964
• NCBI GeneID: 3814
• NCBI Accession #: NP_002247.3
• NCBI GenBank Nucleotide #: NM_002256.3
• Molecular Weight: 14,705 Da
• NCBI Official Full Name: metastasis-suppressor KiSS-1 preproprotein
• NCBI Official Synonym Full Names: KiSS-1 metastasis-suppressor
• NCBI Official Symbol: KISS1
• NCBI Official Synonym Symbols: HH13; KiSS-1
• NCBI Protein Information: metastasis-suppressor KiSS-1
• UniProt Protein Name: Metastasis-suppressor KiSS-1
• Protein Family: Metastasis-suppressor
• UniProt Gene Name: KISS1
• UniProt Entry Name: KISS1_HUMAN

NCBI Description

This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may inhibit chemotaxis and invasion and thereby attenuate metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A protein product of this gene, kisspeptin, stimulates gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulates the pubertal activation of GnRH nuerons. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues. [provided by RefSeq, Mar 2012]

Uniprot Description

KiSS-1: Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell- matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine- tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Belongs to the KISS1 family.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: apical plasma membrane; cell soma; cytosol; extracellular region; extracellular space; neuron projection

Molecular Function: kisspeptin receptor binding; protein binding

Biological Process: cytoskeleton organization and biogenesis; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); generation of ovulation cycle rhythm; negative regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of luteinizing hormone secretion; positive regulation of MAPKKK cascade; positive regulation of synaptic transmission

Disease: Hypogonadotropic Hypogonadism 13 With Or Without Anosmia