Rabbit anti-Human GPR120 Polyclonal Antibody | anti-GPR120 antibody

GPR120, ID (O3FAR1, GPR120, GPR129, PGR4, Omega-3 fatty acid receptor 1, G-protein coupled receptor 120, G-protein coupled receptor 129, G-protein coupled receptor GT01, G-protein coupled receptor PGR4) (APC)

Cat. Num. AAA6303679

• Gene Names: FFAR4; GT01; PGR4; BMIQ10; GPR120; GPR129; O3FAR1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPR120; Polyclonal Antibody; ID (O3FAR1; GPR129; PGR4; Omega-3 fatty acid receptor 1; G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4) (APC); O3FAR1; G-protein coupled receptor PGR4; anti-GPR120 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-GPR120 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GPR120 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-260 amino acids from the Central region of human GPR120.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GPR120 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GPR120 antibody detected the GPR120 protein (arrow).)

Related Product Information for anti-GPR120 antibody

GPR120 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al., 2003 [PubMed 14623098]).

Product Categories/Family for anti-GPR120 antibody

Antibodies; G Protein Receptors

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Burns, R.N., et al. Biochem. Biophys. Res. Commun. 396(4):1030-1035(2010). Moore, K., et al. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 154(4):419-426(2009). Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005). Hirasawa, A., et al. Nat. Med. 11(1):90-94(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 55953085
• NCBI GeneID: 338557
• NCBI Accession #: NP_859529.2
• NCBI GenBank Nucleotide #: NM_181745.3
• UniProt Accession #: Q5NUL3; Q495H1; Q5VY25; Q5VY26; Q7Z605; Q86SM7
• Molecular Weight: 40,494 Da
• NCBI Official Full Name: free fatty acid receptor 4 isoform GPR120-L
• NCBI Official Synonym Full Names: free fatty acid receptor 4
• NCBI Official Symbol: FFAR4
• NCBI Official Synonym Symbols: GT01; PGR4; BMIQ10; GPR120; GPR129; O3FAR1
• NCBI Protein Information: free fatty acid receptor 4; omega-3 fatty acid receptor 1; G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4
• UniProt Protein Name: Free fatty acid receptor 4
• UniProt Gene Name: FFAR4
• UniProt Synonym Gene Names: GPR120; GPR129; O3FAR1; PGR4
• UniProt Entry Name: FFAR4_HUMAN

NCBI Description

This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

GPR120: Receptor for medium and long-chain free fatty acid (FAA). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti- inflammatory effects particularly in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1 dependent effect but independent of G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediate the taste of fatty acids. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Lipid-binding; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: endocytic vesicle; integral to plasma membrane; plasma membrane

Molecular Function: peptide binding; fatty acid binding; taste receptor activity

Biological Process: hormone secretion; G-protein coupled receptor protein signaling pathway; negative regulation of inflammatory response; negative regulation of cytokine secretion; detection of chemical stimulus involved in sensory perception of taste; negative regulation of apoptosis; cellular response to hormone stimulus

Disease: Body Mass Index Quantitative Trait Locus 10