Mouse Phospholipase A2, Group IIA (PLA2G2A) ELISA Kit | PLA2G2A elisa kit

Mouse Phospholipase A2, Group IIA (PLA2G2A) ELISA Kit

Cat. Num. AAA453746

• Gene Names: Pla2g2a; EF; Mom1; Pla2; sPLA2; sPla2-IIA
• Reactivity: Mouse
• Synonyms: Phospholipase A2; Group IIA (PLA2G2A); Mouse Phospholipase A2; Group IIA (PLA2G2A) ELISA Kit; PLA2; MOM1; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2(platelets; synovial fluid); Non-pancreatic secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A; PLA2G2A elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of PLA2G2A. No significant cross-reactivity or interference between PLA2G2A and analogues was observed.
• Sequence Length: 146

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 5.7pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLA2G2A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLA2G2A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLA2G2A elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PLA2G2A in mouse serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLA2G2A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PLA2G2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLA2G2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLA2G2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 132626633
• NCBI GeneID: 18780
• NCBI Accession #: NP_001076000.1
• NCBI GenBank Nucleotide #: NM_001082531.1
• UniProt Accession #: P31482; Q60871
• Molecular Weight: 16,145 Da
• NCBI Official Full Name: phospholipase A2, membrane associated
• NCBI Official Synonym Full Names: phospholipase A2, group IIA (platelets, synovial fluid)
• NCBI Official Symbol: Pla2g2a
• NCBI Official Synonym Symbols: EF; Mom1; Pla2; sPLA2; sPla2-IIA
• NCBI Protein Information: phospholipase A2, membrane associated
• UniProt Protein Name: Phospholipase A2, membrane associated
• Protein Family: Phospholipase
• UniProt Gene Name: Pla2g2a
• UniProt Synonym Gene Names: EF

NCBI Description

Proteins belonging to the phospholipase A2 (PLA2) family hydrolyze phospholipids into sn2 fatty acids and lysophospholipids. They function in a variety of cellular processes, including the digestion of phospholipids and the production of molecules that induce inflammatory responses. This gene encodes a member of the group II class of secretory PLA2s. The secreted enzyme binds to heparin on the cell surface. Mutations in this gene increase the occurrence of intestinal polyps caused by a dominant mutation in the adenomatosis polyposis coli gene. A frameshift inactivates this gene product in some mouse strains including the strain of the reference genome, C57BL/6J, whereas a functional protein is produced in other strains. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:8425615). Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB. Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner ().