Human Phospholipase A2, Group IIA (PLA2G2A) RTU ELISA Kit | PLA2G2A rtu elisa kit

Human Phospholipase A2, Group IIA (PLA2G2A) ELISA Kit

Cat. Num. AAA4501893

• Gene Names: PLA2G2A; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• Reactivity: Human
• Synonyms: Phospholipase A2; Group IIA (PLA2G2A); Human Phospholipase A2; Group IIA (PLA2G2A) ELISA Kit; PLA2; MOM1; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2(platelets; synovial fluid); Non-pancreatic secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A; PLA2G2A rtu elisa kit

• Reactivity: Human
• Sequence Length: 144

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 6.25-400pg/mL
• Sensitivity: 2.55pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLA2G2A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLA2G2A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PLA2G2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLA2G2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PLA2G2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505849
• NCBI GeneID: 5320
• NCBI Accession #: NP_000291.1
• NCBI GenBank Nucleotide #: NM_000300.3
• UniProt Accession #: P14555; Q6DN24; Q6IBD9; Q9UCD2; A8K5I7
• Molecular Weight: 16,083 Da
• NCBI Official Full Name: phospholipase A2, membrane associated
• NCBI Official Synonym Full Names: phospholipase A2 group IIA
• NCBI Official Symbol: PLA2G2A
• NCBI Official Synonym Symbols: MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• NCBI Protein Information: phospholipase A2, membrane associated
• UniProt Protein Name: Phospholipase A2, membrane associated
• Protein Family: Phospholipase
• UniProt Gene Name: PLA2G2A
• UniProt Synonym Gene Names: PLA2B; PLA2L; RASF-A; NPS-PLA2

NCBI Description

The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:2925633). Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins (PubMed:18635536, PubMed:25398877). Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 (PubMed:18635536, PubMed:25398877). Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:25398877). Induces cell proliferation in an integrin-dependent manner (PubMed:18635536).