Human Phospholipase A2, Group IIA (PLA2G2A) ELISA Kit | PLA2G2A elisa kit

Human Phospholipase A2, Group IIA (PLA2G2A) ELISA Kit

Cat. Num. AAA2018845

• Gene Names: PLA2G2A; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• Reactivity: Human
• Synonyms: Phospholipase A2; Group IIA (PLA2G2A); Human Phospholipase A2; Group IIA (PLA2G2A) ELISA Kit; PLA2; MOM1; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2(platelets; synovial fluid); Non-pancreatic secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A; PLA2G2A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Group IIA (PLA2G2A).; No significant cross-reactivity or interference between Phospholipase A2, Group IIA (PLA2G2A) and analogues was observed.
• Sequence Length: 144

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 12.5-800pg/mL
• Sensitivity: < 5.1pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Group IIA (PLA2G2A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Group IIA (PLA2G2A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLA2G2A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group IIA (PLA2G2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group IIA (PLA2G2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group IIA (PLA2G2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Group IIA (PLA2G2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PLA2G2A elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 13543521
• NCBI GeneID: 5320
• UniProt Accession #: P14555; Q6DN24; Q6IBD9; Q9UCD2; A8K5I7
• Molecular Weight: 16,083 Da
• NCBI Official Full Name: Phospholipase A2, group IIA (platelets, synovial fluid)
• NCBI Official Synonym Full Names: phospholipase A2, group IIA (platelets, synovial fluid)
• NCBI Official Symbol: PLA2G2A
• NCBI Official Synonym Symbols: MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• NCBI Protein Information: phospholipase A2, membrane associated
• UniProt Protein Name: Phospholipase A2, membrane associated
• Protein Family: Phospholipase
• UniProt Gene Name: PLA2G2A
• UniProt Synonym Gene Names: PLA2B; PLA2L; RASF-A; NPS-PLA2
• UniProt Entry Name: PA2GA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

PLA2G2A: Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.

Protein type: Secreted, signal peptide; Lipid Metabolism - alpha-linolenic acid; Mitochondrial; Phospholipase; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Lipid Metabolism - arachidonic acid; EC 3.1.1.4; Endoplasmic reticulum; Vesicle; Secreted; Lipid Metabolism - linoleic acid

Chromosomal Location of Human Ortholog: 1p35

Cellular Component: extracellular space; endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; extracellular region; secretory granule

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; phospholipid binding; calcium ion binding

Biological Process: defense response to Gram-positive bacterium; somatic stem cell maintenance; phosphatidic acid metabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; lipid catabolic process; positive regulation of inflammatory response; negative regulation of epithelial cell proliferation

Disease: Colorectal Cancer