Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) Standard Curve.)
Mouse Phosphatidylinositol 3-Kinase Regulatory Subunit beta (PIK3R2) ELISA Kit | PIK3R2 elisa kit
Mouse Phosphatidylinositol 3-Kinase Regulatory Subunit beta (PIK3R2) ELISA Kit
Gene Names
Pik3r2; p85beta
Reactivity
Mouse
Synonyms
Phosphatidylinositol 3-Kinase Regulatory Subunit beta (PIK3R2); Mouse Phosphatidylinositol 3-Kinase Regulatory Subunit beta (PIK3R2) ELISA Kit; PIK3R2; P85B; p85; p85-BETA; phosphatidylinositol 3-kinase; regulatory subunit; polypeptide 2 (p85 beta); phosphoinositide-3-kinase; regulatory subunit 2 (p85 beta); regulatory subu; PIK3R2 elisa kit
Reactivity
Mouse
Specificity
Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PIK3R2. No significant cross-reactivity or interference between Mouse PIK3R2 and analogues was observed.
Sequence Length
722
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) Standard Curve.)
Related Product Information for PIK3R2 elisa kit
Description: This Mouse Phosphatidylinositol 3-kinase regulatory subunit beta (PIK3R2) ELISA Kit employs a two-site sandwich ELISA to quantitate PIK3R2 in samples. An antibody specific for PIK3R2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIK3R2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3R2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3R2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3-prime position. The products of these reactions serve as second messengers in growth signaling pathways. The kinase itself is made up of a catalytic subunit of molecular mass 110 kD (PIK3CA) and a regulatory subunit of either 85 kD, 55 kD, or 50 kD. Both p85-alpha and p85-beta bound Xbp1s and increased its nuclear translocation, and it appeared that the p110 PI3K catalytic subunit and Xbp1s competed for binding of these regulatory subunits. p85-alpha and p85-beta formed an inactive dimer that was disrupted by insulin in a time-dependent manner, which promoted their association with Xbp1s.
Background: Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3-prime position. The products of these reactions serve as second messengers in growth signaling pathways. The kinase itself is made up of a catalytic subunit of molecular mass 110 kD (PIK3CA) and a regulatory subunit of either 85 kD, 55 kD, or 50 kD. Both p85-alpha and p85-beta bound Xbp1s and increased its nuclear translocation, and it appeared that the p110 PI3K catalytic subunit and Xbp1s competed for binding of these regulatory subunits. p85-alpha and p85-beta formed an inactive dimer that was disrupted by insulin in a time-dependent manner, which promoted their association with Xbp1s.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphatidylinositol 3-kinase regulatory subunit beta
NCBI Official Synonym Full Names
phosphoinositide-3-kinase regulatory subunit 2
NCBI Official Symbol
Pik3r2
NCBI Official Synonym Symbols
p85beta
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit beta
UniProt Protein Name
Phosphatidylinositol 3-kinase regulatory subunit beta
Protein Family
UniProt Gene Name
Pik3r2
UniProt Synonym Gene Names
PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta
UniProt Entry Name
P85B_MOUSE
Research Articles on PIK3R2
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Product Notes
The Mouse PIK3R2 pik3r2 (Catalog #AAA9717358) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717358 ELISA Kit recognizes Mouse PIK3R2. It is sometimes possible for the material contained within the vial of "Phosphatidylinositol 3-Kinase Regulatory Subunit beta (PIK3R2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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