Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) Standard Curve.)
Mouse Phosphatidylinositol-4, 5-bisphosphate 3-Kinase Catalytic Subunit delta Isoform (PIK3CD) ELISA Kit | PIK3CD elisa kit
Mouse Phosphatidylinositol-4, 5-bisphosphate 3-Kinase Catalytic Subunit delta Isoform (PIK3CD) ELISA Kit
Gene Names
Pik3cd; AW545373; p110delta; 2410099E07Rik; 2610208K16Rik
Reactivity
Mouse
Synonyms
Phosphatidylinositol-4; 5-bisphosphate 3-Kinase Catalytic Subunit delta Isoform (PIK3CD); Mouse Phosphatidylinositol-4; 5-bisphosphate 3-Kinase Catalytic Subunit delta Isoform (PIK3CD) ELISA Kit; PIK3CD; P110DELTA; PI3K; p110D; PI3-kinase p110 subunit delta; catalytic phosphatidylinositol 3-kinase delta; phosphatidylinositol-4; 5-bisphosphate 3-kinase catalytic subunit delta; phosphoinositide-3-kinase C; PIK3CD elisa kit
Reactivity
Mouse
Specificity
Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PIK3CD. No significant cross-reactivity or interference between Mouse PIK3CD and analogues was observed.
Sequence Length
1043
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) Standard Curve.)
Related Product Information for PIK3CD elisa kit
Description: This Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) ELISA Kit employs a two-site sandwich ELISA to quantitate PIK3CD in samples. An antibody specific for PIK3CD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIK3CD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3CD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3CD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110-alpha, p110-beta, and p110-gamma. p110-alpha and p110-beta interact with SH2/SH3-domain-containing p85 adaptor proteins and with GTP-bound Ras. Like p110-alpha and p110-beta, p110-delta binds p85 adaptor proteins and GTP-bound Ras. These 3 class I PI3Ks were indistinguishable at the level of p85 adaptor protein selection or recruitment to activated receptor complexes. However, unlike p110-alpha, p110-delta does not phosphorylate p85, but instead has an autophosphorylation activity.
Background: Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110-alpha, p110-beta, and p110-gamma. p110-alpha and p110-beta interact with SH2/SH3-domain-containing p85 adaptor proteins and with GTP-bound Ras. Like p110-alpha and p110-beta, p110-delta binds p85 adaptor proteins and GTP-bound Ras. These 3 class I PI3Ks were indistinguishable at the level of p85 adaptor protein selection or recruitment to activated receptor complexes. However, unlike p110-alpha, p110-delta does not phosphorylate p85, but instead has an autophosphorylation activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform isoform a
NCBI Official Synonym Full Names
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
NCBI Official Symbol
Pik3cd
NCBI Official Synonym Symbols
AW545373; p110delta; 2410099E07Rik; 2610208K16Rik
NCBI Protein Information
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
UniProt Protein Name
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
Protein Family
UniProt Gene Name
Pik3cd
UniProt Synonym Gene Names
PI3-kinase subunit delta; PI3K-delta; PI3Kdelta; PtdIns-3-kinase subunit delta; PtdIns-3-kinase subunit p110-delta; p110delta
UniProt Entry Name
PK3CD_MOUSE
Research Articles on PIK3CD
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Product Notes
The Mouse PIK3CD pik3cd (Catalog #AAA9717359) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717359 ELISA Kit recognizes Mouse PIK3CD. It is sometimes possible for the material contained within the vial of "Phosphatidylinositol-4, 5-bisphosphate 3-Kinase Catalytic Subunit delta Isoform (PIK3CD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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