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Typical Testing Data/Standard Curve (for reference only)

Mouse Peptide YY (PYY) ELISA Kit | PYY elisa kit

Mouse Peptide YY (PYY) ELISA Kit

Gene Names
PYY; PYY1; PYY-I
Reactivity
Mouse
Synonyms
Peptide YY (PYY); Mouse Peptide YY (PYY) ELISA Kit; PYY1; Peptide tyrosine tyrosine; PYY elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Peptide YY (PYY).
No significant cross-reactivity or interference between Peptide YY (PYY) and analogues was observed.
Sequence Length
97
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
12.35-1,000pg/mL
Sensitivity
< 4.52pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Peptide YY (PYY) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Peptide YY (PYY) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PYY elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Peptide YY (PYY) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Peptide YY (PYY) and unlabeled Peptide YY (PYY) (Standards or samples) with the pre-coated antibody specific to Peptide YY (PYY). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Peptide YY (PYY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Peptide YY (PYY) in the sample.
Product Categories/Family for PYY elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,360 Da
NCBI Official Full Name
peptide YY preproprotein
NCBI Official Synonym Full Names
peptide YY
NCBI Official Symbol
PYY
NCBI Official Synonym Symbols
PYY1; PYY-I
NCBI Protein Information
peptide YY
UniProt Protein Name
Peptide YY
Protein Family
UniProt Gene Name
PYY
UniProt Synonym Gene Names
PYY
UniProt Entry Name
PYY_HUMAN

NCBI Description

The protein encoded by this gene is proteolytically processed to release a peptide that inhibits pancreatic secretion and mobility in the gut. Rare variations in this gene could increase susceptibility to obesity. [provided by RefSeq, Jul 2010]

Uniprot Description

PYY: This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. Belongs to the NPY family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: extracellular space; extracellular region

Molecular Function: neuropeptide hormone activity; protein binding; G-protein-coupled receptor binding; hormone activity

Biological Process: cell proliferation; G-protein coupled receptor protein signaling pathway; cell-cell signaling; neuropeptide signaling pathway; eating behavior; regulation of appetite; digestion; feeding behavior; cytoskeleton organization and biogenesis; cell motility

Disease: Obesity

Research Articles on PYY

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Product Notes

The Mouse PYY pyy (Catalog #AAA2021208) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2021208 ELISA Kit recognizes Mouse PYY. It is sometimes possible for the material contained within the vial of "Peptide YY (PYY), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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