Oncostatin M/OSM Active Protein | OSM active protein

Oncostatin M/OSM Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122537

• Gene Names: Osm; OncoM
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Oncostatin M/OSM; Oncostatin M/OSM Protein; Mouse; Recombinant (His Tag); Mouse Oncostatin M/OSM Protein (His Tag); OncoM Protein; oncostatin M; OSM active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg206

• Species: Mouse
• Activity: 1. Measured by its ability to inhibit proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 50-200ng/mL.; 2. Measured in a cell proliferation assay using NIH3T3 cells. The ED50 for this effect is typically 2-12ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asn 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mature form of mouse OSM (P53347)(Met 1-Arg 206) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured in a cell proliferation assay using NIH3T3 cells. The ED50 for this effect is typically 2-12 ng/mL.)

Related Product Information for OSM active protein

Background: Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that has functions mainly in cell growth. Oncostatin M (OSM) is considered as a pleiotropic cytokine that signals through cell surface receptors type I and type II both of which share the similarity of containing protein gp130 and takes part in many bio metabolism processes including liver development, hematopoiesis, inflammation, bone formation, and destruction and possibly CNS development. Oncostatin M (OSM) was previously identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported that OSM, like LIF, IL-6, and G-CSF, can inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. The human form of OSM is insensitive between pH2 and 11 and resistant to heating for one hour at 56 degrees but is not stable at 90 degrees. The human OSM is produced as a precursor containing 252 amino acids, whose first 25 amino acids function as a secretory signal peptide and which on removal yields the soluble 227 amino acid pro-OSM. Removal of the C-terminal most 31 amino acids produces the fully active 196 residue form.

References

Tanaka M, et al. (2003) Oncostatin M, a multifunctional cytokine. Rev Physiol Biochem Pharmacol. Reviews of Physiology, Biochemistry and Pharmacology. 149: 39-52.Auguste P, et al. (1997) Signaling of type II oncostatin M receptor. J Biol Chem. 272 (25): 15760-4.Zarling JM, et al. (1986). Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells. Proc Natl Acad Sci. 83 (24): 9739-43.

NCBI and Uniprot Product Information

• NCBI GI #: 61656173
• NCBI GeneID: 18413
• NCBI Accession #: NP_001013383.1
• NCBI GenBank Nucleotide #: NM_001013365.2
• UniProt Accession #: P53347; Q3U1Y5; Q5SPX6
• Molecular Weight: 30,114 Da
• NCBI Official Full Name: oncostatin-M
• NCBI Official Synonym Full Names: oncostatin M
• NCBI Official Symbol: Osm
• NCBI Official Synonym Symbols: OncoM
• NCBI Protein Information: oncostatin-M
• UniProt Protein Name: Oncostatin-M
• Protein Family: Oncostatin
• UniProt Gene Name: Osm
• UniProt Synonym Gene Names: OSM
• UniProt Entry Name: ONCM_MOUSE

Uniprot Description

OSM: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. Belongs to the LIF/OSM family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; oncostatin-M receptor binding; cytokine activity

Biological Process: negative regulation of hormone secretion; tyrosine phosphorylation of Stat3 protein; tyrosine phosphorylation of Stat5 protein; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system development; negative regulation of cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; behavioral response to pain; positive regulation of MAPKKK cascade; response to heat; regulation of growth; negative regulation of meiosis; positive regulation of cell proliferation; tyrosine phosphorylation of Stat1 protein; immune response; positive regulation of transcription from RNA polymerase II promoter